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1

Isolation and cultivation of endosymbiotic alga from green hydra and phylogenetic analysis of 18S rDNA sequences

100%
Kovacevic G., Franjevic D., Jelencic B., Kalafatic M.
Folia Biologica
|
2010
|
vol. 58
|
issue 1-2
135-143
EN
Symbiotic associations are of wide significance in evolution and biodiversity. The green hydra is a typical example of endosymbiosis. In its gastrodermal myoepithelial cells it harbors the individuals of a unicellular green algae. Endosymbiotic algae from green hydra have been successfully isolated and permanently maintained in a stable clean lab culture for the first time.We reconstructed the phylogeny of isolated endosymbiotic algae using the 18S rRNA gene to clarify its current status and to validate the traditional inclusion of these endosymbiotic algae within the Chlorella genus. Molecular analyses established that different genera and species of unicellular green algae could be present as symbionts in green hydra, depending on the natural habitat of a particular strain of green hydra.
2

Karyological analysis of two allopatric populations of planarian Polycelis felina (Daly.) in Croatia

76%
Kalafatic M., Kopjar N., Zrna G., Zupan I., Kovacevic G., Franjevic D.
Folia Biologica
|
2004
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vol. 52
|
issue 1-2
131-133
EN
The results of preliminary karyological investigations of two geographically distant and morphologically slightly different Polycelis felina (Daly.) populations from central Croatia are reported. The results have shown that individuals of both populations are diploids, with the same chromosome number in their neoblasts (2n=18). Their karyotypes were composed of nine chromosome pairs, three of them are metacentric and the other six are submetacentric. Statistical evaluation of data indicated that both populations of Polycelis felina (Daly.), despite minor differences observed between them, belong to the same karyological biotype.
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