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1

Localisation of rDNA in the Lupinus genome during the cell cycle

100%
Naganowska B., Zielinska A.
Journal of Applied Genetics
|
2004
|
vol. 45
|
issue 2
189-193
EN
Observations of a specific rDNA locus behaviour during the cell cycle were made by fluorescent in situ hybridisation (FISH) in 12 Lupinus species. Due to the pattern of chromatin de-condensation in that locus, the number of relevant sites in interphase nuclei was twice as high as the number of signals on metaphase chromosomes. The description of successive phases and an attempt of an explanation are given.
2

Chromosomes of Lupinus hispanicus subsp. hispanicus Boiss. et Reut., L. luteus L. and their hybrids

100%
Naganowska B., Ladon D.
Journal of Applied Genetics
|
2000
|
vol. 41
|
issue 3
167-170
EN
Cytological observations of mitotic chromosomes were performed for two species and interspecific hybrid lines: Lupinus hispanicus subsp. hispanicus (line Badajoz 2, cat. no. Wt 96383), L. luteus cv. Topaz (cat. no. Wt 98006), L. ? hispanicoluteus (cat. no. Wt 98301 and Wt 98302). It was found that L. hispanicus, L. luteus and L. ? hispanicoluteus have the same chromosome number 2n = 52. Chromosome morphology is, generally, similar. Centromeres are located mostly in median or submedian region. Chromosome differentiation appears to be very poor, except the first (the longest) pair which is easily discernible in both parental species and in the hybrid.
3

Karyotyping of the narrow-leafed lupin (Lupinus angustifolius L.) by using FISH, PRINS and computer measurements of chromosomes

81%
Kaczmarek A., Naganowska B., Wolko B.
Journal of Applied Genetics
|
2009
|
vol. 50
|
issue 2
77-82
EN
BAC (bacterial artificial chromosome) clones from the genomic BAC library of the narrow-leafed lupin (Lupinus angustifolius) were used for cytogenetic mapping of mitotic metaphase chromosomes of that species by the BAC-FISH technique. Location of the clones, together with cytogenetic markers localised earlier by FISH (fluorescence in situ hybridisation) and PRINS (primed in situ DNA labelling), was combined with computer-aided chromosome measurements, to construct the first idiogram of the narrow-leafed lupin. The chromosomes are meta- or submetacentric; the mean absolute chromosome lengths range from 1.9 mum to 3.8 mum, and mean relative lengths from 1.6% to 3.3%. Data concerning linkage of resistance to 2 fungal pathogens as well as assignment of the second linkage group to the appropriate chromosome are given for the first time.
4

Meiosis and fertility of reciprocal triploid hybrids of Lolium multiflorum with Festuca pratensis

71%
Naganowska B., Zwierzykowski Z. Z., Zwierzykowski Z., Zwierzykowska Z. E., Zwierzykowska E.
Journal of Applied Genetics
|
2001
|
vol. 42
|
issue 3
247-255
EN
Abstract. Diploid and tetraploid forms of Lolium multiflorum and Festuca pratensis were crossed under controlled conditions and after embryo rescue all four combinations of autoallotriploid hybrids were obtained. Male and female fertility and chromosome pairing at metaphase I of meiosis were studied in several plants from each hybrid combination. The hybrids with two genomes of L. multiflorum and one of F. pratensis (genomic formulae LmLmFp and FpLmLm) were male and female fertile while the hybrids with two genomes of F. pratensis and one of L. multiflorum had a reduced fertility (FpFpLm) or were completely sterile (LmFpFp). Chromosome pairing at metaphase I varied among hybrid combinations depending on their genomic composition. LmLmFp and FpLmLm hybrids had similar patterns of pairing (1.83I + 5.29II + 2.85III and 2.22I + 5.22II + 2.75III, respectively) but they differed from those of FpFpLm (3.65I + 4.65II + 2.68III) and especially from LmFpFp (4.78I + 5.87II + 1.49III). Conventional analysis of meiosis failed to explain the differences in chromosome behaviour and fertility/sterility levels between the autoallotriploid hybrids with two Lolium or two Festuca genomes.
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