Full-text resources of PSJD and other databases are now available in the new Library of Science.
Visit https://bibliotekanauki.pl
Preferences help
Polish version English version Language
enabled [disable] Abstract
Number of results

Results found: 12

Number of results on page
first rewind previous Page / 1 next fast forward last

Search results

help Sort By:

help Limit search:
first rewind previous Page / 1 next fast forward last
1

Increase of genetic variability by somatic hybridization in Gramineae

100%
Rybczynski J. J.
Biotechnologia
|
2006
|
issue 4
136-144
EN
The paper reviews somatic hybridization of grasses and cereals. The following subjects are be discussed: 1) methods of somatic embryo production, 2) levels and methods used for somatic hybrids' description 3) species participating in hybridization programs, 4) traits used for selection of somatic hybrids in in vitro culture, 5) agricultural traits searched in particular combination of hybridization, 6) ploidy of somatic regenerants and their isoenzymatic variation.
2

Present status of biotechnology of rye (Secale cereale L.)

64%
Bolesta E., Rybczynski J. J.
Biotechnologia
|
2001
|
issue 4
55-73
EN
This review paper covers the results of tissue culture and biotechnology of rye (Secale cereale L.) published between 1990 and 2000. The following subjects were raised: somatic embryogenesis, haploid production, intergenetic hybridisation and transformation. Upon conclusion of all the results published to date, we may say that the progress of biotechnology in the case of rye is very limited in comparison to other cereals. However, a lot of work and efforts were involved to obtain the aforementioned result. Taking into account the progress in plant genetics and molecular biology, the authors deeply believe that the New Millennium will bring a brake-through in rye biotechnology.
3

Cryopreservation of suspended cells of selected Gentiana

64%
Mikula A., Rybczynski J. J.
Biotechnologia
|
2003
|
issue 3
39-51
EN
Cryopreservation is recognised as the most efficient method of plant genome preservation and embryogenic potential of plant in vitro cultures with non-changed status. Because of the risk of biological degradation of tissue during freezing treatment and it various sensibility on the chilling stress, the individual treatment with cryoprotectance and parameters of the temperature decreasing is highly required. In this presentation, following subjects will be discussed: 1) description of suspension cultures of various gentians, 2) cell pretreatments for surviving the stress of low temperature, 3) rearrangement of cell cytoplasm because of high sucrose concentrations, 4) the role of sucrose in the development of freezing tolerance and surviving of gentaiana suspensions, 5) the effect of strong plasmolysis induced by 3 M glucose.
4

Morphogenic potential of plant cell and its genetic conditions

64%
Rybczynski J. J., Mikula A.
Biotechnologia
|
2000
|
issue 2
41-54
EN
The aim of the presented review is to analyse the possibilities of creating highly morphogenic Triticum aestivum genome using generative hybridisation of various wheat forms and its relatives.
5

Plant regeneration from immature inflorescence-derived callus of Italian ryegrass (Lolium multiflorum Lam.): chromosome number and fertility of regenerated plants

64%
Zwierzykowski Z., Rybczynski J. J.
Journal of Applied Genetics
|
1998
|
vol. 39
|
issue 3
217-224
EN
Twenty field-grown genotypes of diploid Italian ryegrass (Lolium multiflorum Lam., 2n = 2x = 14) were tested for their ability to induce callus and regenerate plants. Callus cultures were initiated from segments of immature inflorescences cultured on the MS medium supplemented with 4.0 mg L-1 2,4-D. The calluses were subcultured first on the maintaining medium (MS medium with 2.0 mg L-1 2,4-D) and later on the rooting medium (MS medium with 0.2 mg L-1 2,4-D). The frequency of callus induction varied depending on the source of explant and the initial genotype. A total of 473 green plantlets were regenerated, of which 420 were established in the soil. All these plants had the morphological characteristics of Italian ryegrass. Among 372 regenerants analysed cytologically, 302 (81.2%) had the expected diploid chromosome number (2n = 2x = 14), 65 (17.5%) were tetraploid (2n = 4x = 28); several aneuploids and mixoploids were also observed. All diploid and tetraploid regenerants were male and female fertile. However, a great variation of female fertility within and between both groups of regenerants was observed.
6

Application of modern methods for plant material studies of Gentiana kurroo (Royle) somatic embryogenesis

64%
Fiuk A., Rybczynski J. J.
Biotechnologia
|
2006
|
issue 4
95-106
EN
Nowadays somatic embryogenesis is being investigated with special attention paid to the identification of genes directly involved in triggering of cell competence and development of ontogenic stages. In order to trace successive cell divisions that lead to the formation of the globular structures, modern scanning and electron microscopy methods are applied. Plantlets, which come from somatic embryogenesis process, should be exactly the same as mother plants but in vitro culture conditions may induce many disturbances, which could be lasting (hereditary) or only transitory. These changes are usually called somaclonal variation and could be observed on different levels of the plant organization. To investigate this kind of variations both of the genetic and epigenetic types, specially designed molecular systems are needed. Here, we describe induction of somatic embryos from several explants of G. kurroo. In order to evaluate the particular ontogenic stages of somatic embryos and variability of regenerated plants, following methods were applied: light microscopy, scanning and transmission electron microscopy, 2D protein electrophoresis, flow cytometry of DNA content in the cell nucleus, cytogenetic analysis of chromosome number and molecular analysis with the use of AFLP.
7

Present status of Gentiana taxa biotechnology

64%
Mikula A., Rybczynski J. J.
Biotechnologia
|
1999
|
issue 4
76-91
EN
Data on the tissue culture and genetic manipulation of several species of the genus Gentiana in support on the review of literature are presented.
8

Cryopreservation - a tool for long-term storage of cells, tissues and organs from in vitro culture derived

64%
Mikula A., Rybczynski J. J.
Biotechnologia
|
2006
|
issue 4
145-163
EN
Cryopreservation offers the possibility for long-term storage of genetic resources with maximal genotypic stability, using a minimum of space and maintenance. At present it is actively used all over the world for storage of plant material: seeds, pollen, spores, dormant buds or apical meristems in genebanks. The development of biotechnology led to the production of a new category of germplasm for cryostorage: in vitro obtain tissues, organs, embryos, special cell lines and genetically modified plant material. The maintenance of in vitro collections remains risky regarding losing accessions due to the contamination, human error or somaclonal variation. The classical slow cooling was the first standard protocol developed for hydrated plant tissues. This method is mainly used for cryopreservation of non-organized tissues, for example: cell suspensions and calli, or apices of cold-tolerant species. For differentiated structures, new cryopreservation techniques such as vitrification and encapsulation/dehydration procedures or droplet method are efficient and reliable. These freezing techniques have been successfully, routinely applied for cryopresevation of various plant material of temperate and tropical climate species. So far, cryopreservation procedures are developed for in vitro tissues and recalcitrant seeds of about 100 and 40 species, respectively.
9

The application of spor culture in vegetative propagation of tree ferns of Cyathea genus

64%
Goller K., Rybczynski J. J.
Biotechnologia
|
2001
|
issue 2
139-143
EN
Multiplication of tree ferns using the traditional methods, i.e. the sowing of spores, is a very difficult task, characterised by low efficiency. It seems that in vitro culture of gamatophyte could be employed to overcome these difficulties. Experiments with vegetative propagation of tree ferns have been carried out in our lab over the last few years. The following species C. australis (R.Br), C. brownii (Domin), C. capensis, C. dealbata (G.Forst.)SW., C. dregei Kunze , C. giganthea, C. medullaris, C. smithii Hook f., C. spinulosa, Cyathea sp Sm were the subjects of our studies. Experiments were carried out with the use of two basal agar media: Murashige and Skoog (1962) and Knop medium. Various concentrations of minerals of the studied media were used. It was proved that the time required for the germination of sown spores on agar medium depended on the species and concentration of used mineral ingredients of the studied media. Finally, numerous plants were obtained only from C. australis and C. Cooperi.
10

Micropropagation of some Lupinus species from seedling explants

64%
Rybczynski J. J., Podyma E.
Genetica Polonica
|
1993
|
vol. 34
|
issue 3
237-247
EN
Experiments were carried out on (cvs. Wat and Hetman).L.luteus (cvs. Topaz and Iryd), (cv, Remik), and . For culture initiation 5- and 14-day-old seedling explants were used. The of the was stimulated by various media but the universal one appeared to be Gamborg's B5 medium solidified with Gelrite. For each investigated species a medium assuring 100% rooting of explants was developed. The most effective combination of growth substances for multiplication of L. hispanicus was found. Cotyledonary node explants, cultured on medium supplemented with benzyladenine and with or without naphthaleneacetic acid were stimulated to undergo in 4 and 2, for L. hispanicus and L. albus, respectively. Plantlets obtained from all experiments were easily potted.
11

Preliminary studies of plant regeneration via somatic embryogenesis induced on immature cotyledons of white lupin (Lupinus albus L.

64%
Rybczynski J. J., Podyma E.
Genetica Polonica
|
1993
|
vol. 34
|
issue 3
249-257
EN
coconut water. Somatic embryos were formed on the surface of cotyledons of immature embryos. The process of was observed only on cotyledons of whole explants. Unfortunately, cotyledons isolated from the embryo axis did not produce somatic embryos. In the subepidermal cell layer, the initial cell divisions leading to somatic embryo formation were observed. The number of regenerated somatic embryos varied from one to many per cotyledon. Somatic embryo derived plantlets formed very good root system in perlite supplemented with medium. Cytological and anatomical evidences of this process are presented in this paper.
12

In vitro culture of Gentiana kurroo (Royle)

51%
Fiuk A., Rajkiewicz M., Rybczynski J. J.
Biotechnologia
|
2003
|
issue 3
267-274
EN
The aim of the presented paper is to show the present status of in vitro studies of Gentiana kurroo (Royle). Experimental material originated from seedlings, organs of regenerants, callus tissue, cell suspensions and isolated protoplasts. Two years of experiments proved very high morphological potential of plant material originated from various system of cultures. The mostly used media were those based on Murashige and Skoog medium, but for protoplast cultures, Kao and Michaluk medium appeared to be most useful. Numerous plant growth regulators with various concentrations and combinations controled plant regeneration of studied gentiana. Plant regeneration via somatic embriogenesis was observed in the majority of the studied culture systems.
first rewind previous Page / 1 next fast forward last
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.