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Roślinne transportery błonowe metali śladowych

100%
Kosmos
|
2013
|
vol. 62
|
issue 1
105-113
PL
Metale śladowe obecne w roztworze glebowym można podzielić na dwie grupy: metale konieczne do prawidłowego funkcjonowania komórek roślinnych (m.in. Fe, Cu, Mn, Co, Zn, Ni) oraz metale niekonieczne (np. Cd, Hg, Pb, Cr). Obecne w roślinach białka transportujące niezbędne metale z gleby do korzenia i dalej do pędu charakteryzują się szeroką specyficznością, stanowiąc drogę wnikania także dla metali niekoniecznych. Utrzymywanie homeostazy poprzez kontrolowanie pobierania, transportu, wypompowywania i sekwestracji tych pierwiastków jest kluczowe dla prawidłowego działania organizmów. Praca ta została poświęcona krótkiej charakterystyce zidentyfikowanych do tej pory roślinnych transporterów błonowych, należących do następujących rodzin białek: ZIP, YSL, NRAMP, P1B-ATP-az, transporterów ABC, COPT, CAX, CDF i IREG.
EN
Trace metals present in soil can be divided into two groups: essential metals indispensable for proper functioning of plant cells (e.g. Fe, Cu, Mn, Co, Zn, Ni), and non-essential metals (e.g. Cd, Hg, Pb, Cr). Transporters for essential trace metals in plants usually have a wide specificity and can equally transport non-essential metals. Maintaining homeostasis by controlling the uptake, transport, removal and sequestration of metals is crucial for organisms. This work is dedicated to a short characterization of identified plant membrane transporters, belonging to several protein families: ZIP, YSL, NRAMP, P1B-ATP-ases, ABC transporters, COPT, CAX, CDF and IREG.
EN
The presence of the single metals (Cd, Pb, Cu, Zn) induces ROS (reactive oxygen species) production and causes oxidative stress in plants. While applied in two-element combinations, trace metals impact organisms in a more complex way. To assess the resultant effect we treated the pea grown hydroponically with the trace metals in variants: CuPb, CuCd, CuZn, PbCd, ZnPb, ZnCd in concentrations of 25 µM for each metal ion. Abiotic stress inhibited root elongation growth, decreased biomass production, induced changes in root colour and morphology. It changed rate of ROS production, malondialdehyde content, increased activity and altered gene expression of defence enzymes (superoxide dysmutase, catalase, ascorbate peroxidase, glutathione reductase, γ-glutamylcysteine synthetase).
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