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1

The use of cell lines and the methods of cell cycle synchronization in mammalian cloning

100%
Modlinski J., Karasiewicz J.
Biotechnologia
|
1998
|
issue 2
86-100
EN
Studies aimed at improving the effectiveness of cloning by nuclear transfer have shown that proper development of the majority of reconstituted embryos is secured by G1 phase of the donor nucleus or by using 'universal recipients' i.e. enucleated pre-activated oocytes. Donor nuclei for cloning may by derived from cell lines of embryonic stem cells (mouse), embryonic cells after short in vitro culture (sheep, pig, cattle) or even from fetal cells or adult cells after in vitro culture and inducing quiescence in their nuclei (sheep). The use of fetal cells for transgenesis in vitro and the production of transgenic sheep after nuclear transfer from these cells opens the way to profitable technology of cloning transgenic farm animals.
2

Multiple generational cloning of livestock embryos

100%
Piotrowska K., Modlinski J.
Biotechnologia
|
1998
|
issue 2
130-136
EN
The main purpose of nuclear transfer in domestic species is to produce a large number of identical animals. There are two main ways to produce clones by nuclear transfer. One is to use the ICM and ED cells cultured under special conditions, as donor nuclei. The other way is to use the nuclear transfer embryo itself as the donor for the next generation of cloning (multiple generational cloning). The in vitro and in vivo developmental ability of nuclear transferred embryos is the same in the case of the first three generations. A limited number of multiple-generation clones were transferred into recipient heifers, resulting in offspring from I, II and III generation clones. The strategy to increase the efficiency of multiple generational bovine embryo cloning is discussed as well as the possible use of rabbit embryos as an experimental model.
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