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A study on influence of different signs of air-ions on sister-chromatid exchange frequency and chromosome aberrations in human peripheral blood lymphocytes

100%
Wiszniewski A., Kretowicz T.
Journal of Applied Genetics
|
1999
|
vol. 40
|
issue 2
129-134
EN
A cytogenetic analysis of peripheral blood lymphocytes of six persons (seven in analysis of chromosome aberrations (CA)) was carried out. The cell cultures were exposed to a constant concentration of positive or negative ions. The exposure time was established as 24 and 72 hours for ions of both signs. In the cultures exposed to positive ions and used for sister-chromatid exchange (SCE) analysis no metaphases were detected. An analysis of cultures after 72 hours of exposition to negative ions revealed an increase in the number of SCE and inhibition of cellular divisions as compared to the control. In CA analysis no differences in the number of aberrations were observed but in cultures exposed to positive ions dicentric chromosomes were detected. Therefore we conclude that the different signs of air-ions had not genotoxic effect.
2

Genetic differences among several species of Tricladida from the relict Lake Ohrid as revealed by enzyme electrophoresis

71%
Sywula T., Krstanovski Z., Tasevska O., Sell J., Kretowicz T.
Folia Biologica
|
2003
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vol. 51
|
issue 1-2
105-109
EN
Six endemic and two widely distributed species living in Lake Ohrid were studied. In general, these hermaphroditic animals displayed no signs of departure from Hardy-Weinberg equilibrium. Genetic variation in all but one of the endemic species was of the same extent as that in geographically wide ranging invertebrates. On the other hand, the Lake Ohrid population of the common European species Dendrocoelum lacteum was monomorphic at all loci examined. D. sanctinaumi, one of the endemic species, exhibited a clear genetic subdivision into spring and littoral subpopulations. The genetic differentiation of Crenobia alpina alpina and C. a. montenigrina proved commensurable to that of well separated species from other genera. The data suggest that the separation of particular lineages in the set of Lake Ohrid endemics was widely dispersed over time.
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