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Serological classification and epitope specificity of Proteus penneri S29 lipopolysaccharide

100%

Zych K., Siwinska M., Sidorczyk Z.

Archivum Immunologiae et Therapiae Experimentalis

2005

vol. 53

issue 6

540-545

Gram-negative bacteria of genus Proteus are common human intestinal and urinary tract pathogens. In the genus Proteus there are four clinically important named species: P. mirabilis, P. vulgaris, P. penneri, and P. hauseri, and three unnamed Proteus genomospecies: 4, 5, and 6. The clinical significance of P. penneri, described in 1982 as a new species, is poorly documented. The aim of this work is serological characterization and classification of a ceftriaxone-susceptible P. penneri S29 strain isolated from a 34-year-old patient with postneurosurgical meningitis. In this characterization we will also include a ceftriaxon-resistant strain, P. penneri R15, isolated from the same patient after 12 days' treatment with ceftriaxon and other antibiotics. Rabbit polyclonal O-antisera were obtained against these two strains and purified lipopolysaccharides (LPS) were extracted from the bacterial mass of the P. penneri S29 and R15 strains. In the serological investigations the following tests were used: enzyme immunosorbent assay (EIA), passive immunohemolysis (PIH), inhibition of these tests, absorption of rabbit O-antisera with the respective LPS, and repeated PIH, SDS/PAGE, and Western blot techniques. The serological studies of the LPS extracted from both P. penneri strains showed the identity of both preparations of O-polysaccharides from LPS.. In P. penneri S29 O-antiserum, four different types of antibodies were described and characterized. Both investigated P. penneri S29 and R15 strains were classified to the Proteus O31ab serogroup.

Characterization and serological classification of O-specific polysaccharide of Proteus mirabilis TG 276-90 from Proteus serogroup O34

88%

Kolodziejska K., Siwinska M., Zych K., Rozalski A., Sidorczyk Z.

Archivum Immunologiae et Therapiae Experimentalis

2006

vol. 54

issue 3

223-226

Introduction: Gram-negative bacteria of the genus Proteus from the family Enterobacteriaceae are currently divided into the five species P. mirabilis, P. vulgaris, P. penneri, P. hauseri, and P. myxofaciens and three unnamed Proteus genomospecies 4, 5, and 6. They are important facultative human and animal pathogens which, under favorable conditions, cause mainly intestinal and urinary tract infections, sometimes leading to serious complications such as acute or chronic pyelonephritis and the formation of bladder and kidney stones. In this study we report on the serological properties of the lipopolysaccharide (LPS) of Proteus mirabilis TG 276-90, whose O-polysaccharide chemical structure was described earlier. Materials and Methods: LPS and alkali-treated LPS of a few serologically related Proteus strains and O-antisera against P. mirabilis TG 276-90 and CCUG 4669 (O34) were used. Serological characterization of P. mirabilis TG 276-90 O-specific polysaccharide was done using enzyme immunosorbent assay, passive immunohemolysis test (PIH), inhibition of these tests, SDS/PAGE and Western blot techniques, absorption of rabbit polyclonal O-antisera, and repeated PIH test. Results: Structural and serological investigations showed that the O-polysaccharides of P. mirabilis TG 276-90 and P. vulgaris O34 are identical and that their LPSs differ only in epitopes in the core part. Therefore these two strains could be classified into the same Proteus O34 serogroup. Conclusions: The serological data showed that the beta-D-GalpNAc-(14)-alpha-D-GalpNAc disaccharide is an important epitope of the P. mirabilis TG 276-90 and P. vulgaris O34 LPSs, shared by the P. mirabilis O16 and P. vulgaris TG 251 LPSs. It is responsible for cross-reactions with P. mirabilis TG 276-90 and P. vulgaris O34 O-antisera.

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2 Archivum Immunologiae et Therapiae Experimentalis

1 2006

1 2005

2 Sidorczyk Z.

2 Siwinska M.

2 Zych K.

1 Kolodziejska K.

1 Rozalski A.

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Serological classification and epitope specificity of Proteus penneri S29 lipopolysaccharide

Characterization and serological classification of O-specific polysaccharide of Proteus mirabilis TG 276-90 from Proteus serogroup O34