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Cavity Enhanced Absorption Spectroscopy Sensor

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The paper presents opportunities of cavity enhanced absorption spectroscopy technique application in nitrogen oxides (NO_{x}) detection. In this method the concentration of an absorbing gas is determined by measure of decay time of the light pulse trapped in an optical cavity. The measurements are not sensitive to fluctuation of both laser power and photodetector sensitivity. The cavity enhanced absorption spectroscopy technique is a modification of cavity ring down spectroscopy technique where the off-axis adjustment of the resonator is used. NO_{x} detection is carried out in the visible and infrared range. The signal is registered with a developed low noise photoreceiver. Features of the presented sensor show that it is possible to build a portable trace gases sensor. Its sensitivity could be comparable with chemical detectors. Such a system has several advantages: relatively low price, small size and weight, and detection possibility of other gases.
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EN
While studying the influence of ionizing radiation or certain chemical agents on cells, it is crucial to not only determine cytotoxicity, but also to follow cell death mechanisms. There are different methods to screen processes of cell death and still very important question remains unanswered about differences in results that could be caused by various experimental steps in procedures. Based on literature review two protocols of cell death determination were compared. First protocol regarded collecting cells floating in medium before trypsinization and following centrifugation of them. In the second protocol floating cells were discarded and attached ones were stained and fixed. In all experiments three different untreated cell lines (A172, DU145 as cancer cell lines and in comparison, fibroblasts (FB CCL 110), as a non- cancerous cell line) were used to test applied protocols. Cells were cultured and death processes were examined at different time points up to 120 h. Compared protocols showed statistically significant differences, especially in terms of necrosis, which was higher when included floating cells from culture medium and then centrifuging them. Therefore, presented results show importance of choosing a valid experimental procedure in case of evaluating cells viability and types of cell death pathways quantitatively.
EN
The paper presents analysis and preliminary investigations of quantum cascade lasers for free space optics. The lasers radiate in the long wavelength IR spectral range (8-12 μm). Because of lower effects of radiation scattering than in the case of 1.5 μm free space optics, better transmission range can be obtained. The main task of the work was experimental investigation of quantum cascade lasers taking into consideration free space optics applications. In the research, quantum cascade lasers operating in both pulse and continuous wave modes were used. The lasers spectra as well as the operation characteristics (e.g. voltage vs. current) were measured. In conclusion, the quantum cascade lasers parameters for free space optics system applications are summarized.
EN
The paper presents the construction and test results of a long-term radiation detector module for free space optical communication operated at the wavelength range of 8-12 μm. In the module a Polish detector manufactured in the company Vigo System S.A. was applied. High sensitivity of the detection module was achieved through a multi-layered heterostructure HgCdTe with immersion lens, which is optimized for the radiation detection at the wavelength of 10 μm. Detector noises were reduced as a result of detector cooling by means of four-stage thermoelectric cooler. The developed detection module is dedicated to design a next-generation optical data link. The link will be characterized by a greater range in difficult weather conditions in relation to the currently offered links operating at the shorter wavelengths.
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