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The effects of captan and captafol on different bacterial strains and on c-mitosis in V79 Chinese hamster fibroblasts

100%

Rahden-Staroń I., Szumiło M., Ziemkiewicz P.

Acta Biochimica Polonica

1994

vol. 41

issue 1

45-55

Defective Brca2 influences topoisomerase I activity in mammalian cells*.

88%

Rahden-Staroń I., Szumiło M., Grosicka E., Zwet M. K. v. d., Zdzienicka M. Z.

2003

vol. 50

issue 1

139-144

The Chinese hamster cell mutant V-C8 is defective in the Brca2 gene (Kraakman-van der Zwet et al., 2002, Cell Biol.; 22: 669). Here we report that V-C8 cells were 10-fold more sensitive to camptothecin, an inhibitor of topoisomerase I, than the parental V79 cells. The level of the relaxation activity of topoisomerase I in nuclear extracts was also lower (4-fold) in V-C8 than V79 cells, in spite of the fact that the level of the topoisomerase I protein was the same in these cells. The survival of V-C8 cells in the presence of camptothecin, the sensitivity of V-C8 topoisomerase I to camptothecin, and the level of the relaxation activity in V-C8 nuclear extract were almost completely restored by transfection of V-C8 cells with the murine Brca2 gene or by the transfer of human chromosome 13 providing the BRCA2 gene. These results indicate that the observed changes in the topoisomerase I activity in V-C8 are due to the defective function of the Brca2 gene.

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2 Acta Biochimica Polonica

1 2003

1 1994

2 Rahden-Staroń I.

2 Szumiło M.

1 Grosicka E.

1 Zdzienicka M. Z.

1 Ziemkiewicz P.

1 Zwet M. K. v. d.

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The effects of captan and captafol on different bacterial strains and on c-mitosis in V79 Chinese hamster fibroblasts

Defective Brca2 influences topoisomerase I activity in mammalian cells*.