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Antioxidant capacity of crude extracts containing carotenoids from the berries of various cultivars of Sea buckthorn (Hippophae rhamnoides L.)

100%
Kruczek M., Świderski A., Mech-Nowak A., Król K.
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2012
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vol. 59
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issue 1
135-137
EN
Comparative analysis of antioxidant capacity was performed using FRAP and DPPH methods on extracts containing carotenoids acquired from fruits of Sea buckthorn. The examination included nine varieties of Sea buckthorn growing in the comparative cultivation. Conducted analysis allowed to compare the antioxidant capacity with carotenoids content measured with spectrophotometric and HPLC methods. Three of the examined cultivars indicating high antioxidant activity in both, FRAP and DPPH methods, also revealed highest ('Aromatnaya') and high ('Botanicheskaya', 'Arumnyj') total carotenoids content in HPLC analysis.
2
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Content of carotenoids in roots of seventeen cultivars of Daucus carota L.

88%
Mech-Nowak A., Świderski A., Kruczek M., Łuczak I., Kostecka-Gugała A.
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2012
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vol. 59
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issue 1
139-141
EN
The aim of this study was to compare the content of carotenoids in seventeen cultivars of carrots grown in Poland. Conventional orange cultivars with rarely grown were compared: white, yellow and purple with yellow core cultivars. To determine the content of carotenoids, extracts from lyophilized carrot roots were prepared and analyzed by spectrophotometric as well as HPLC methods with DAD detector. The highest content of carotenoids was found in cultivars: 'Korund F1' (48 mg/100g of fresh weight) and 'Salsa F1' (36 mg/100g of fresh weight). The antioxidant properties of selected cultivars were compared using the DPPH method.
3
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Callitriche cophocarpa - a new rich source of active phenolic compounds

76%
Augustynowicz J., Długosz-Grochowska O., Kostecka-Gugała A., Leja M., Kruczek M., Świderski A.
Open Chemistry
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2014
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vol. 12
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issue 4
519-527
EN
This study was focused on the analysis of the contents (by HPLC-DAD) and antioxidant activity of phenolic compounds of aquatic higher plant Callitriche cophocarpa. The novelty of the work was: 1) the study of the species which is almost unknown in the context of investigations of bio-active compounds and, 2) the application of a unique L-band electron resonance spectroscopy (L-band EPR) to measure antioxidant activity of samples. The results were compared with those obtained for microalgae Chlorella and Spirulina which are widely distributed as the diet supplements. The obtained results are very promising with respect to the freeze-dried material of Callitriche, which revealed both high level of phenolic compounds (33 mg g−1 dry weight) and antioxidant activity (0.14 g of reduced DPPH· g−1 dry weight). An isoform of sinapic acid appeared to be the main fraction in this sample. The average content of the total phenolic compounds in Callitriche freeze-dried samples was ca. 43 or 25 times higher than in the case of the Chlorella or Spirulina samples, respectively. These samples showed also 16 times lower scavenging activity in relation to freeze-dried Callitriche material.
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