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  1. 2 Biotechnologia

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  1. 1 2010

  2. 1 2004

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  1. 2 Czubacka A.

  2. 2 Doroszewska T.

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1

Obtaining double haploid transgenic tobacco lines by the tissue culture method

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Czubacka A., Doroszewska T.
Biotechnologia
|
2004
|
issue 2
37-45
EN
In the study on double haploid (DH) lines of tobacco, two cultivars ? MN944 and BY103 transformed with a construct carrying the coat protein gene of Lettuce Mosaic Virus (LMV) CP were used. The hybrids F1 from crossing transgenic line BY103 with its non-transformed equivalent as well as transgenic MN944 line with cv. Wi?lica were used for producing haploids via inducted androgenesis. Double haploids were received by regeneration from stem fragments. The course of meiosis in pollen mother cells was analysed and pollen viability was estimated.
2

Resistance of transgenic tobacco lines to Potato Virus Y

100%
Czubacka A., Doroszewska T.
Biotechnologia
|
2010
|
issue 2
72-82
EN
Potato virus Y (PVY) causes a disease which reduces the quality of raw material as well as the yield. This study on resistance included tobacco transgenic lines belonging to four cultivars modified with three genetic constructions, containing Lettuce mosaic virus coat protein gene (LMV CP) and PVY replicase gene used in sense (ROKY1) and antisense (ROKY2) orientation. The plants were inoculated with PVY isolates under greenhouse conditions and tested by DAS-ELISA. The highest effectiveness of antiviral protection was noticed in case of the lines with transgene LMV CP, lower for ROKY2, and the lowest for ROKY1. In following generations, the percentage increase of a number of plants resistant to PVY was observed within the lines containing transgene LMV CP. Cultivar MN 944 showed to be the best genetic background for that transgene, while cultivar AC Gayed for transgene ROKY.
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