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Comparison of Electroporation Threshold for Different Cell Lines in vitro

100%
Saulis G., Saulė R.
Acta Physica Polonica A
|
2009
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vol. 115
|
issue 6
1056-1058
EN
The electroporation threshold was compared at various electric pulse durations for three cell lines: two non-tumor cell lines (human erythrocytes and Chinese hamster ovary cells) and one tumor cell line (rat glioma C6 cells). First, the dependences of the fraction of electroporated cells on the pulse intensity were obtained for the cells exposed to single square-wave electric pulses with the durations of 0.02-2 ms. Then, the average cell radii were measured for each cell line and the transmembrane potential induced by the external electric field was calculated. The obtained values of the transmembrane potential were in the range of 480-930 mV and decreased with increasing pulse duration. The obtained dependences of the transmembrane potential required to electroporate 50% of cells on the pulse duration were close to each other for all cell lines studied.
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High-Voltage Pulses Potentiate Bleomycin Cytotoxicity Towards Cancer Cells in vitro

81%
Saulė R., Saulis G., Batiuškaitė D.
Acta Physica Polonica A
|
2009
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vol. 115
|
issue 6
1098-1100
EN
In this study the influence of high-voltage pulses on bleomycin cytotoxicity towards mouse hepatoma MH-22A and rat glioma C6 cells in vitro was determined. It was obtained that combining bleomycin with pulses of strong electric field significantly enhances the number of cells killed by bleomycin. Bleomycin alone was cytotoxic to both cell lines at concentrations above 1 μM. Treatment of cells by electric pulses in the presence of bleomycin greatly potentiated its cytotoxicity towards both cell lines tested - the bleomycin concentration required to reduce cell survival by 50% was 3.5-3.8 nM only. Exposure of cells to electric pulses only did not reduce cell viability.
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Changes of the Electrode Surface Roughness Induced by High-Voltage Electric Pulses as Revealed by AFM

81%
Rodaitė-Riševičienė R., Saulis G., Snitka V.
Acta Physica Polonica A
|
2009
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vol. 115
|
issue 6
1095-1097
EN
The changes of the surface topography of stainless-steel and aluminium electrodes occurring due to the action of electric pulses which are utilized for cell electroporation, have been studied by using atomic force microscopy. The surfaces of the polished stainless-steel electrodes were smooth - the average roughness was 13-17 nm and the total roughness 140-180 nm. The total roughness of the aluminium electrodes was about 320 nm. After the treatment of the chambers filled with 154 mM NaCl solution by a series of short (20-40 μs), high-voltage (4 kV) pulses with the total dissolution charge of 0.20-0.26 A s/cm^{2}, the roughness of the surface of the electrodes has increased, depending on the total amount of the electric charge that has passed through the unit area of the electrode. Up to a two- and threefold increase of the surface roughness of the stainless-steel and aluminium anodes respectively was observed due to the dissolution of the anode material. Therefore, the use of high-voltage electric pulses leads to the increase of the inhomogeneity of the electric field at the electrode, which facilitates the occurrence of the electric breakdown of the liquid samples and causes non-equal treatment of each cell.
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