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RT-PCR-based identification of genetic variants of alpha S1 casein in milking goats

100%

Tokarska M., Szumska D., Dobosz T.

Journal of Applied Genetics

1999

vol. 40

issue 4

335-342

Genetic variants of the alphaS1 casein gene in goats determine the different casein and protein content of milk, variant milk processing quality and cheese flavour. The main seven alleles of alphaS1 casein belong to four groups: strong alleles, which code for a high alphaS1 content (3.6 g/L), medium alleles (1.6 g/L), weak alleles (0.6 g/L) and allele 0 (complete lack of alphaS1 casein). An original method was designed, based on PCR with three specific primers and mRNA isolated from milk somatic cells. The length of PCR products varied due to differences between alleles on cDNA level, since allele F has a 111 nt deletion of 9-11 exons, allele D is associated with deletion of 36 nt (exon 9), and allele E includes a 457 nt retropozon insertion in exon 19. Forty-nine goats coming from four different farms were genotyped. Each of the analysed goats is the carrier of at least one `strong' allele, which codes for the beneficial high level of alphaS1 casein.

Genetic parameters of coat colour in golden fox (Vulpes vulpes L.)

64%

Filistowicz F. A., Filistowicz A., Przysiecki P. P., Przysiecki P., Wierzbicki W. H., Wierzbicki H., Tokarska T. M., Tokarska M.

Journal of Applied Genetics

2000

vol. 41

issue 4

259-265

Genetic parameters (heritability, genetic and phenotypic correlations) of chosen coat colour traits of golden fox were estimated. 1013 animals, born on the ?niaty fox farm (Poland) in 1985-1999 were evaluated. In 1993-1999 colour type was additionally assessed for 833 animals, by detailed evaluation of coat colour on the back and sides of the body, throat colour, belly colour and the amount of silver hair. The REML method was used to estimate genetic (co)variance components. Data were transformed using the probit transformation. Heritability estimates for coat traits were low (0.04 to 0.22). Values of most of the estimated genetic parameters (h2, rG, rP) were comparable to those frequently reported for other colour types of silver fox.

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2 Journal of Applied Genetics

1 2000

1 1999

2 Tokarska M.

1 Dobosz T.

1 Filistowicz A.

1 Filistowicz F. A.

1 Przysiecki P.

1 Przysiecki P. P.

1 Szumska D.

1 Tokarska T. M.

1 Wierzbicki H.

1 Wierzbicki W. H.

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RT-PCR-based identification of genetic variants of alpha S1 casein in milking goats

Genetic parameters of coat colour in golden fox (Vulpes vulpes L.)