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A survey of satelite association formation by human acrocentric chromosomes depending on their Ag-NOR class

100%

Trzepizur K., Hubner H., Trzepizur Z.

Journal of Applied Genetics

1995

vol. 36

issue 1

69-80

The frequency of individual Ag-NOR classes acc.to classification of Spinner et al.in the chromosomes of 37 woman and 63 men and the frequency of satellite association (SA) formation by acrocentric chromosomes depending on Ag-NOR class were studied.Totally, 6686 satelite associations formed by 14730 acrocentric chromosomes and by 35270 acrocentric chromosomes beyond SA were surveyed.It was inferred that chromosomes 12 and 22, which are smaller than chromosomes 13-15, possess more extended nucleolus organizer regions.The frequency of Ag-NOR clesses in chromosomes is not depended on sex.It was found that acrocentric chromosomes with Ag-NOR classes I, II and III are more frequently encountered in SA, whereas those with Ag-NOR classes IV and V are more frequent in the metaphase plate beyond SA.Acrocentric chromosomes 21 and 22 with Ag-NOR classes I and II reside in SA equally frequently.Comparisons between chromosomes 21(22)-13, 21(22)-14 and 21(22)-15 show that chromosomes 21 and 22 more frequently enter into SA than chromosomes of group 13-15.No direct relations were found between the degree of NOR silver-staining of an acrocentric chromosome and a more frequent entry of this chromosome into SA or into a definite type of SA.

Analysis of chromosome aberrations, sister chromatid exchanges (SCE) and cell division kinetics in human lymphocytes exposed in vitro to new monophosphates of pyrimidine acyclonucleosides

88%

Ferenc T., Rutkowski M., Bratkowska W., Hubner H., Draminski M.

Journal of Applied Genetics

1998

vol. 39

issue 1

113-127

Five newly synthesised monophosphates of two pyrimidine acyclonucleoside series, namely 1-N-[(2'-hydroxy)ethoxymethyl] and 1-N-[(1',3'-dihydroxy)-2'-propoxymethyl] derivatives of 5- and 5,6-alkylated uracils were tested in vitro for chromosome aberrations and sister chromatid exchanges (SCE). Metaphase plates were obtained via microculture of human lymphocytes from heparinized peripheral blood. The compounds were tested in doses: 10, 20, 40, 80 and 150 ?g per mL of culture. The tested compounds induced mainly chromatid gaps, less frequently chromosome gaps. A low number of mitoses with chromatid and chromosome breaks, acentric fragments, dicentric chromosomes and exchange figures were also observed. The tested compounds in doses: 40, 80 and 150 ?g per mL, doubled or tripled the percentage of cells with chromatid gaps and chromosome gaps as compared to the control. The percentage of cells with aberrations (excluding gaps) induced by the tested compounds in all doses did not exceed 2%. The tested compounds induced a higher number of SCE per cell but less than double frequency as compared to the control. SCE frequencies and replication index (RI) values varied depending on the examined compounds. For the highest dose of the tested compounds (150 ? per mL) a significant decrease in RI values was observed for 1-N-[(2'-hydroxy)ethoxymethyl]-5,6-tetramethyleneuracil monophosphate and for 1-N-[(2'-hydroxy)ethoxymethyl] -5,6-dimethyluracil monophosphate. So far, the results have indicated potential clastogenicity of all the tested compounds except acycloguanosine monophosphate.

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2 Journal of Applied Genetics

1 1998

1 1995

2 Hubner H.

1 Bratkowska W.

1 Draminski M.

1 Ferenc T.

1 Rutkowski M.

1 Trzepizur K.

1 Trzepizur Z.

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A survey of satelite association formation by human acrocentric chromosomes depending on their Ag-NOR class

Analysis of chromosome aberrations, sister chromatid exchanges (SCE) and cell division kinetics in human lymphocytes exposed in vitro to new monophosphates of pyrimidine acyclonucleosides