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The bovine tyrosine hydroxylase gene associates in vitro with the nuclear matrix by its first intron sequence*.;

100%

Lenartowski R., Grzybowski T., Miścicka-Śliwka D., Wojciechowski W., Goc A.

Acta Biochimica Polonica

2003

vol. 50

issue 3

865-873

Recently we have shown that in vitro binding of the proximal part of the human tyrosine hydroxylase gene to the nuclear matrix is correlated with its transcriptional activity. The strongest binding potential was predicted by computing for the first intron sequence (Lenartowski & Goc, 2002, Neurosci Lett.; 330 : 151-154). In this study a 16 kb fragment of the bovine genomic DNA containing the tyrosine hydroxylase gene was investigated for its affinity to the nuclear matrix. Only a 950 bp fragment encoding the distal part of the first intron, second exon and a few nucleotides of the second intron bound to the nuclear matrix. The binding was independent of the tissue-specific tyrosine hydroxylase gene activation. The fragment was subcloned and sequenced. Computer search pointed to one potential intronic matrix attachment region with two AP1-like sites embedded in the sequence. We conclude that even if the position of the matrix binding region is conserved among the tyrosine hydroxylase genes in mammals, its tissue specificity and/or function is not preserved or is achieved by different mechanisms.

Optimization of the Y831C mutation detection in human DNA polymerase gamma by allelic discrimination assay

100%

Stopińska K., Grzybowski T., Malyarchuk B. A., Derenko M. V., Miścicka-Śliwka D.

Acta Biochimica Polonica

2006

vol. 53

issue 3

591-595

Many well-defined mutations in the gene for the catalytic subunit of polymerase γ (POLG1) have been found to be associated with disease, whereas the status of several mutations remains unresolved due to the conflicting reports on their frequencies in populations of healthy individuals. Here, we have developed a highly sensitive, real-time allelic discrimination assay enabling detection of the Y831C mutation in the POLG1 gene. The Y831C mutation is present in the Polish population at a frequency of 2.25%. The new assay is well suited to both extensive population studies and molecular diagnostics of POLG1.

Analysis of microsatellite instability and loss of heterozygosity in breast cancer with the use of a well characterized multiplex system.

73%

Powierska-Czarny J., Miścicka-Śliwka D., Czarny J., Grzybowski T., Woźniak M., Drewa G., Czechowicz W., Sir J.

Acta Biochimica Polonica

2003

vol. 50

issue 4

1195-1203

Analysis of microsatellite instability (MI) and loss of heterozygosity (LOH) is recommended for screening patients with sporadic and hereditary malignancies. This study shows an application of a fluorescent hexaplex PCR system for microsatellite typing on A.L.F. DNA Sequencer (Pharmacia Biotech). This technique detects changes in microsatellites providing a time-efficient, reliable and accurate method for MI and LOH analyses. The Fragment Manager software was used for automated size calculation and quantitation of DNA fragments, enabling rapid and precise measurement of allelic ratios. We examined 70 breast cancer and 70 control DNA specimens, classified all the patterns of microsatellite alterations, and set up MI and LOH assessment criteria for the automated multiplex fluorescent method.

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3 Acta Biochimica Polonica

1 2006

2 2003

3 Grzybowski T.

3 Miścicka-Śliwka D.

1 Czarny J.

1 Czechowicz W.

1 Derenko M. V.

1 Drewa G.

1 Goc A.

1 Lenartowski R.

1 Malyarchuk B. A.

1 Powierska-Czarny J.

1 Sir J.

1 Stopińska K.

1 Wojciechowski W.

1 Woźniak M.

Search results

The bovine tyrosine hydroxylase gene associates in vitro with the nuclear matrix by its first intron sequence*.;

Optimization of the Y831C mutation detection in human DNA polymerase gamma by allelic discrimination assay

Analysis of microsatellite instability and loss of heterozygosity in breast cancer with the use of a well characterized multiplex system.