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In vitro culture of isolated embryos and ovules from wide crosses within the genus brassica

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Wojciechowski A., Maslankiewicz J., Chen Y., Kwapiszewska M., Springer B.

Biotechnologia

2001

issue 2

86-89

In vitro culture of hybrid embryos and ovules from crosses of B. campestris, B. oleracea, B. fruticulosa and B. napus were conducted on White, Murashige and Skoog, Murashige and Skoog modified by Keller and Nitsh and Nitsh media. The highest efficiency of crosses was obtained in the crosses B. campestris x B. oleracea, and the lowest in crosses with B. fruticulosa. In cultures of 572 embryos from 11 cross combinations, plants were regenerated from 24,5% of cultured embryos. From 3 cross combinations 502 ovules were placed on the medium. In this case the plants were regenerated from 2 of 3 crosses and the efficiency of the culture was over 46%.

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1 Biotechnologia

1 2001

1 Chen Y.

1 Kwapiszewska M.

1 Maslankiewicz J.

1 Springer B.

1 Wojciechowski A.

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In vitro culture of isolated embryos and ovules from wide crosses within the genus brassica