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1

Nonaqueous media ? site of enzymatic catalysis

100%
Bancerz R., Ginalska G.
Biotechnologia
|
2009
|
issue 4
168-182
EN
Lipases, esterases, acylases, glycosidases, aldolases are the most used enzymes in synthetic organic chemistry. Among the biocatalysts, lipases are the most frequently used. This class of enzymes is able to catalyze the hydrolysis of carboxylic acid esters in aqueous medium on the reverse reaction in organic solvents. The use of enzymes in organic solvents is now well established and there are several advantages of conducting enzymatic reaction in water poor media such as increased solubility of hydrophobic substrates, shifting of thermodynamic equilibrium in favour of synthesis over hydrolysis and increased thermostability of the enzyme. However, the practical application of enzyme in organic solvents has been hampered by the low catalytic activities compared to those expressed in water. This review highlights recent research on the stabilization of enzymes using both chemical and biological means to increase the lifetime o the biocatalyst.
2

The supports for enzymes immobilization and their applications

100%
Lobarzewski J., Ginalska G.
Biotechnologia
|
1994
|
issue 1
44-63
3

The application of immobilized invertase for continuous production of inverted sucrose

81%
Ginalska G., Belcarz A., Lobarzewski J., Wolski T.
Biotechnologia
|
1999
|
issue 1
226-237
EN
Four inorganic supports (sand, silica gel, crushed pumice and controlled-pore glass) were used for immobilization of invertase. The possibility of application of thus obtained immobilized enzyme in continuous production of inverted sugar was tested. The best support for immobilization of invertase was controlled-pore glass (CPG, average pore size of 31 mm). The conditions of production of inverted sugar by immobilized enzyme were optimised (also in column). Unter the optimised the column (dimensions: 0.7x27 cm) with immobilized invertase was able to degrade sucrose to glucose and fructose in an amount of 60 g/h. Immobilized invertase in the column remained fully active for 120 days.
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