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Antimicrobial activity of petroleum ether and methanolic extracts from fruits of Seseli devenyense Simonk. and the herb of Peucedanum luxurians Tamam.

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Widelski J., Grzegorczyk A., Malm A., Chinou I., Głowniak K.
Current Issues in Pharmacy and Medical Sciences
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2015
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vol. 28
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issue 4
257-259
EN
Plants of the Apiaceae family usually contain coumarins. These are used worldwide in traditional medicine, as well as in modern therapeutics. The aim of our study was to determine the antimicrobial activity of four extracts (methanolic and petroleum ether extracts) obtained from two Apiaceae species: Seseli devenyense (fruits) and Peucedanum luxurians (herb). The activity of the investigated extracts was tested against 7 strains of Gram+ bacteria, and 7 strains of Gram-, as well as three of yeast. The results of this show that the best activity of such extracts (specifically, by way of petroleum ether) was seen as being against Staphylococcus aureus strains.
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Influence of extraction methods on the recovery of astragaloside IV from the roots ofAstragalus mongholicusin Soxhlet- and Twisselmann-type apparatus

73%
Kukuła-Koch W., Baj T., Sieniawska E., Koch W., Widelski J., Luchowska K., Kołodziej B., Głowniak K.
Open Chemistry
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2015
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vol. 13
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issue 1
EN
Astragalus mongholicus, commonly used in Traditional Chinese Medicine (TCM), contains significant amounts of triterpene saponins – astragalosides, which possess a wide variety of pharmacological actions, including the treatment of cardiovascular and respiratory diseases. The present study was performed to develop a fast and effective extraction method of saponins from crude plant material using Twisselmann-type apparatus. Moreover, a detailed comparison between Soxhlet and Twisselmann apparatus was performed to explain the advantages and disadvantages of their applications. The content of astragaloside IV – the major saponin of A. mongholicus extracts and chosen as an extraction efficiency marker ‒ was determined by means of RP-HPLCELSD chromatography in an herein optimized method. Twisselmann extraction shortened its duration from 4 to 2 hours in comparison with the Soxhlet method ‒ a generally accepted method preparation for this extract. The effectiveness of both apparatus was alike (3.24 ± 0.44 mg and 3.48 ± 0.26 mg of astragaloside IV/ 4g of powdered root for 2 hours in Twisselmann and 4 hours in Soxhlet apparatus). Compared with a pharmacopoeial method, the application of Twisselmann apparatus enabled a decrease in analysis time of 50%, which is of high significance, especially for large, scaled-up industrial scale.
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