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Energy efficiency as a factor of architecture development. Tradition and present

100%

Ostrowska B.

The paper explains how climatic conditions and the closely related principle of energy efficiency influence the forms of traditional buildings, as well as presents how to draw on traditional experiences in shaping the contemporary sustainable architecture. The methods of reducing buildings energy consumption are assigned to three main stages of its existence: the stage of construction, operation and decommissioning.

Targeting the hypoxia pathway in malignant plasma cells by using 17-allylamino-17-demethoxygeldanamycin

33%

Kocemba-Pilarczyk K., Ostrowska B., Trojan S., Aslan E., Kusior D., Lasota M., Lenouvel C., Dulińska-Litewka J.

2018

vol. 65

issue 1

101-109

Multiple myeloma (MM) is characterized as a clonal expansion of malignant plasma cells in the bone marrow, which is often associated with pancytopenia and osteolytic bone disease. Interestingly, myeloma-infiltrated bone marrow is considered to be hypoxic, providing selection pressure for a developing tumour. Since HSP90 was shown to participate in stabilization of the subunit of the key transcription factor HIF-1, which controls the hypoxic response, the aim of this study was to investigate the influence of a HSP90 inhibitor 17-allylamino-17-demethoxygeldanamycin (17-AAG), on MM cells cultured under low oxygenation conditions. We confirmed that 17-AAG inhibits hypoxic induction of the HIF-1 target genes in malignant plasma cells and demonstrate the concentration range of severe hypoxia-specific cytotoxicity. Next, we selected the malignant plasma cells under severe hypoxia/re-oxygenation culture conditions in the presence or absence of 17-AAG and subsequently, the cells which survived were further expanded and analyzed. Interestingly, we have noticed significant changes in the survival and the response to anti-MM drugs between the parental cell lines and those selected in cyclic severe hypoxia in the presence and absence of 17-AAG. Importantly, we also observed that the lack of oxygen itself, irrespectively of HIF-1 inhibition, is the main/pivotal factor driving the selection process in the experiments presented here.

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1 Acta Biochimica Polonica

1 Czasopismo Techniczne

1 2018

1 2015

2 Ostrowska B.

1 Aslan E.

1 Dulińska-Litewka J.

1 Kocemba-Pilarczyk K.

1 Kusior D.

1 Lasota M.

1 Lenouvel C.

1 Trojan S.

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Energy efficiency as a factor of architecture development. Tradition and present

Targeting the hypoxia pathway in malignant plasma cells by using 17-allylamino-17-demethoxygeldanamycin