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1

Introgression of Brassica napus genome into restorer lines for CMS ogura estimated with the use of molecular markers

100%
Poplawska W., Bartkowiak-Broda I.
Biotechnologia
|
2001
|
issue 1
112-118
EN
The object of research were double low restorer lines of CMS ogura. The restorer gene was introgressed into rapeseed genotype from radish (Raphanus sativus) through intergeneric hybridisation. The obtained recombinants of rapeseed with the restorer gene comprise a DNA fragment originating from radish, which is bigger than the locus of the restorer gene. It disturbs the first meiotic behaviour in PMC. In addition, it has an impact on the fertility and yielding ability of restorer lines. Moreover, the restorer gene in the obtained recombinant is tightly linked with a gene responsible for high content of undesirabled compounds in seeds - glucosinolates. Elimination of the unnecessary DNA fragment is performed by backcrosses with double low lines of winter rapeseed. The changes in rapeseed genome which are a result of backcrosses are investigated by the use of molecular and isozyme markers. Key words: Brassica napus, restorer gene, introgression, molecular and isozyme markers. Adres do korespondencji:
2

Progress and status of doubled haploids of winter oilseed rape (Brassic napus L.) production

100%
Cegielska-Taras T., Bartkowiak-Broda I.
Biotechnologia
|
2006
|
issue 4
107-115
EN
A single microspore cultured in vitro can be reprogrammed from the development of pollen to divisions and production of a bipolar embryo. The final effect of androgenesis in vitro is conversion of each embryo derived from microspore of a heterozygous plant into a doubled haploid plant in such a way that a population of doubled haploids fully represents the genetic variability of the preceding meiosis. DH lines have been already widely used in oilseed rape breeding. Doubled haploids are used by breeders in crossing programs in order to obtain desirable variability in progeny, and also as a part of conventional breeding. Moreover, doubled haploids in conjunction with molecular markers helped to develop the so called 'molecular breeding'.
3

The practical use of molecular markers in hybrid breeding of winter oilseed rape (Brassica napus L. var. oleifera)

81%
Poplawska W., Liersch A., Bartkowiak-Broda I.
Biotechnologia
|
2004
|
issue 2
17-22
EN
Breeding of oilseed rape hybrid varieties in Poland is based on CMS ogura hybrydization system. The marker assisted selection is used in selection of parental lines of F1 hybrids. The markers of alleles of restorer gene Rfo are the most important in breeding programs. Also, the investigations on genetic distance of hybrid parental lines using molecular markers are undertaken aiming at its application for preliminary selection of F1 combinations.
4

Microspore culture of winter oilseed rape (Brassica napus L.) in conjunction with other in vitro technologies

81%
Cegielska-Taras T., Szala L., Bartkowiak-Broda I.
Biotechnologia
|
2001
|
issue 1
81-86
EN
Microspore culture in conjuction with other technologies such as selection, mutagenesis and transformation has been used for the production of novel genotypes of Brassica napus L. for crop improvement. The example of in vitro selection of microspore - derived embryos includes: a) ploidy level, b) seed oil composition (for example: high level of erucic acid), c) genotypes with restorer gene for CMS-ogura system (by means of isozyme marker PGI-2 ), d) herbicide resistant forms. Efficiency of microspore mutagnesis has been tested by the treatment of freshly isolated microspores with UV and MNU. Direct delivery of foreign gene to the microspores (microprojectile bombardment) combined with the use of Agrobacterium tumefaciens to microspore derived embryos seems to be a promising way of oilseed rape transformation.
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