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1
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Low molecular mass products of depolymerization of purified mucin - attempts at isolation and characterization

100%
Minkiewicz I., Gindzieński A.
Acta Biochimica Polonica
|
1999
|
vol. 46
|
issue 4
928-933
EN
Samples of crude mucin were incubated at room temperature for 48 and 96 h in a sodium azide containing buffer, pH 7.0. Then each sample was purified, reduced and alkylated with iodo[^14C]acetamide. Electrophoretic analysis demonstrated that radioactivity was incorporated into the mucin subunits and proteins of 100 and 140 kDa. The results of our experiments suggest that the released proteins can be a part of mucin molecule, cleaved by proteolysis and reduction of disulfide bridges.
2
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The participation of ribosome-UDP-GalNAc complex in the initiation of protein glycosylation in vitro

81%
Paszkiewicz-Gadek A., Porowska H., Gindzieński A.
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2000
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vol. 47
|
issue 2
421-426
EN
The gastric epithelial cells ribosome-UDP-GalNAc complex is a donor of UDP- GalNAc as the substrate for N-acetylgalactosaminyltransferase, which catalyse the transfer of GalNAc residue to the polypeptide, existing on polysomes. It was observed that the deglycosylated porcine mucin and synthetic peptide (PTSSPIST) can be also glycosylated with participation of N-acetylgalactosaminyltransferase and ribosome- UDP-GalNAc complex. The probability of the ribosome-UDP-GalNAc complex as an intermediate in the O-glycosylation is considered.
3
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Activity of partially purified UDP-N-acetyl-α-D-galacto- samine:polypeptide N-acetylgalactosaminyltransferase with different peptide acceptors

81%
Porowska H., Paszkiewicz-Gadek A., Gindzieński A.
Acta Biochimica Polonica
|
1999
|
vol. 46
|
issue 2
365-370
4
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Studies on type I collagen in skin fibroblasts cultured from twins with lethal osteogenesis imperfecta.

81%
Galicka A., Wołczyński S., Gindzieński A.
|
2003
|
vol. 50
|
issue 2
481-488
EN
Studies on type I procollagen produced by skin fibroblasts cultured from twins with lethal type II of osteogenesis imperfecta (OI) showed that biosynthesis of collagen (measured by L-[5-3H]proline incorporation into proteins susceptible to the action of bacterial collagenase) was slightly increased as compared to the control healthy infant. SDS/PAGE showed that the fibroblasts synthesized and secreted only normal type I procollagen. Electrophoretic analysis of collagen chains and CNBr peptides showed the same pattern of electrophoretic migration as in the controls. The lack of posttranslational overmodification of the collagen molecule suggested a molecular defect near the amino terminus of the collagen helix. Digestion of OI type I collagen with trypsin at 30°C for 5 min generated a shorter than normal α2 chain which melted at 36°C. Direct sequencing of an asymmetric PCR product revealed a heterozygous single nucleotide change C→G causing a substitution of histidine by aspartic acid in the α2 chain at position 92. Pericellular processing of type I procollagen by the twin's fibroblasts yielded a later appearance of the intermediate pC-α1(I) form as compared with control cells.
5
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EF-1α Is a target site for an inhibitory effect of quercetin in the peptide elongation process

81%
Marcinkiewicz C., Gałasiński W., Gindzieński A.
Acta Biochimica Polonica
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1995
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vol. 42
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issue 3
347-350
6
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Structure and biosynthesis of human salivary mucins.

81%
Zalewska A., Zwierz K., Żółkowski K., Gindzieński A.
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2000
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vol. 47
|
issue 4
1067-1079
EN
Human salivary glands secrete two types of mucins: oligomeric mucin (MG1) with molecular mass above 1 MDa and monomeric mucin (MG2) with molecular mass of 200-250 kDa. Monomers of MG1 and MG2 contain havily O-glycosylated tandem repeats located at the central domain of the molecules. MG1 monomers are linked by disulfide bonds located at sparsely glycosylated N- and C-end. MG1 are synthesized by mucous cells and MG2 by the serous cells of human salivary glands.
7
Content available remote

Purification and characterization of the protein kinase eEF-2 isolated from rat liver cells

80%
Gajko A., Gałasiński W., Gindzieński A.
Acta Biochimica Polonica
|
1994
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vol. 41
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issue 4
421-427
8
Content available remote

Low molecular mass products of depolymerization of purified mucin - attemps of isolation and characterization

80%
Minkiewicz I., Gindzieński A.
Acta Biochimica Polonica
|
1999
|
vol. 46
|
issue 4
929-933
9
Content available remote

Application of aqueous hydrazine solution for β-elimination of O-glycans from gastric mucin

70%
Kisiel D., Gindzieński A., Gadek A.
Acta Biochimica Polonica
|
1999
|
vol. 46
|
issue 3
753-757
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