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Clinical and prognostic value of hTERT mRNA expression in patients with non-small-cell lung cancer

100%

Zalewska-Ziob M., Dobija-Kubica K., Biernacki K., Adamek B., Kasperczyk J., Bruliński K., Ostrowska Z.

2017

vol. 64

issue 4

641-646

Telomerase, undetectable in normal somatic cells, plays a critical role in carcinogenesis of the majority of human tumors including lung carcinoma. The aim of our study was to determine human telomerase reverse transcriptase (hTERT) mRNA expression in patients with non-small cell lung cancer (NSCLC) in order to estimate its usefulness as diagnostic and/or prognostic factor. hTERT expression was analyzed in a group of 12 females and 28 males with NSCLC using Quantitative Real-Time Polymerase Chain Reaction (QRT-PCR method) in cancerous and non-cancerous lung tissues. Results were analyzed according to clinical data and one-, two-, and five-year survival rates. hTERT expression in the cancerous tissue was significantly higher than in the lung parenchyma free from neoplasm infiltration (p<0.05). There was no significant association between hTERT expression in the tumor tissue and age, gender, grading or clinical stage. A significant difference in hTERT expression between two types of histopathological patterns (adenocarcinoma and squamous cell carcinoma) was detected (p=0.01). No association between hTERT expression in NSCLC specimens and survival rates was found. hTERT mRNA detection by QRT-PCR in tumor and corresponding cancer-free tissues can be used as a diagnostic marker in patients with NSCLC, but seems not to be a prognostic factor.

The role of MGMT polymorphisms rs12917 and rs11016879 in head and neck cancer risk and prognosis

84%

Kiczmer P., Prawdzic Seńkowska A., Strzelczyk J., Szydło B., Biernacki K., Osadnik T., Krakowczyk Ł., Ostrowska Z.

2018

vol. 65

issue 1

87-92

Head and neck squamous cell carcinoma (HNSCC) is one of the leading cancers by incidence worldwide. The risk of these cancers is strictly associated with alkylation factors present in tobacco smoke. The crucial role in preventing DNA alkylation is played by O6-methylguanine-DNA methyltransferase (MGMT). Dysfunction or lack of MGMT is associated with an increased risk of cancer. The aim of the study was to assess the influence of MGMT polymorphisms: rs12917 and rs11016879 on HNSCC risk and course. The study consisted of 69 HNSCC patients and 242 healthy individuals. Case samples were taken from resected tumour tissue. The control group comprised samples of epithelial cells collected from mucous membranes using swabs. DNA samples were genotyped by employing the 5' nuclease assay for allelic discrimination using TaqMan SNP Genotyping Assays. The significance between distributions of genotypes and alleles was tested using Pearson's χ2 test analysis. Our results indicated that the MGMT rs12917 TT genotype increases the risk of HNSCC. The MGMT rs11016879 AG genotype and A allele were associated with increased HNSCC risk. We noted higher risk of nodal metastasis in rs11016879 AA homozygotes. Mechanisms leading to MGMT enzymatic defect are unknown and hence further studies need to be carried out. Our data suggest that the examined polymorphisms may be considered as potential prognostic factors for HNSCC risk and outcome. Further studies are necessary to verify our results.

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2 Acta Biochimica Polonica

1 2018

1 2017

2 Biernacki K.

2 Ostrowska Z.

1 Adamek B.

1 Bruliński K.

1 Dobija-Kubica K.

1 Kasperczyk J.

1 Kiczmer P.

1 Krakowczyk Ł.

1 Osadnik T.

1 Prawdzic Seńkowska A.

1 Strzelczyk J.

1 Szydło B.

1 Zalewska-Ziob M.

Search results

Clinical and prognostic value of hTERT mRNA expression in patients with non-small-cell lung cancer

The role of MGMT polymorphisms rs12917 and rs11016879 in head and neck cancer risk and prognosis