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1

Genetic modification of microorganisms for ethanol production from hemicellulosic hydrolyzates

100%
Nowak J.
Biotechnologia
|
1998
|
issue 3
89-97
EN
The efficient and complete conversion of lignocellulosic hydrolysates to ethanol as a result of fermentation processes makes possible the production of a bio-fuel competitive with petroleum. Traditional organisms used in ethanol fermentation, e.g. Saccharomyces cerevisise and Zymomonas mobilis, are unable to ferment pentose sugars. To overcome this problem genetic modifications of S. cerevisiae, Z. mobilis and E. coli were performed in order to produce ethanol directly from xylose and arabinose. The performance of the recombinant strains is encouraging: especially over the last 3 years the progress has been rapid. There is a possibility that strains of these three species may find a separate application in the fermentation of specific plant biomasses.
2

Bioremedation of soil contamined with petroleum products

100%
Nowak J.
Biotechnologia
|
2008
|
issue 1
97-108
EN
Bioremediation is a process by which microoorganisms degrade or transform the environmental contaminants into less toxic forms. A wide variety of bacterial and fungal genera are known to be capable of degrading, and in many cases, completely mineralizing chemical substances present in petroleum products at present. Three types of bioremediation are predominant in the industry: natural attenuation, biostimulation and bioaugmentation. Selecting the most appropriate strategy to treat a specific site can be quided by considering three basic principles: the amenability of the pollutant to biological transformation to less toxic products, the accessibility of contaminant to microorganisms (bioavailabilty) and the opportunity for optimization of biological activity. Microbial activity is affected by a range of environmental factors, including nutrients, moisture content, pH, temperature, and oxygen concentration. Different aspects of bacterial degradation of petroleum contaminants in soil and how to improve the efficiency and reproducibility are discussed in this review.
3
Content available remote

Angular Dependence of Susceptibility in Thin Fe_{78}B_{19}Si_{3} Films

64%
Nowak J., Wenda J.
Acta Physica Polonica A
|
1994
|
vol. 85
|
issue 2
419-422
EN
The differential transversal susceptibility as a function of an external magnetic field was measured for several directions of the magnetic field with respect to the anisotropy axis. Both the anisotropy field and coercive field were determined by analyzing the symmetry properties of the susceptibility field dependence. The Stoner-Wohlfarth model was modified by introducing an internal field and used to fit the experimental data. The hyperbolic parts of susceptibility curves corresponding to single domain states were well reproduced by fitting the appropriate constant value of the internal field.
4

Thermophilic aerobic biodegradation of food industry wastewater

64%
Lasik M., Nowak J.
Biotechnologia
|
2006
|
issue 3
98-112
EN
Food industry wastewater is usually characterized by a high biological load as well as elevated temperature. A new and promising technology for rapid and effective biodegradation of such hot and high loaded wastewater is thermophilic aerobic biodegradation process. It is characterized by higher, in comparison with mesophilic processes, substrate degradation, rapid inactivation of pathogenic microorganisms and low production of activated sludge. High biodegradation rate shortens the time of the process and reduces the required volume of bioreactors. Disadvantages of the aerobic thermophilic process are associated with costs of aeration of bioreactors, low capabilities of thermophilic microflora for flocculation, and problems with foaming during fermentation.
5

The possibility of modeling functional properties of legume food products through the tempeh type fermentation

64%
Kuligowski M., Nowak J.
|
2007
|
issue 4
113-124
EN
Tempeh is a fermented food, originally produced from soy beans, which is characterized by many interesting nutritional, health and functional proprieties. Using various raw material and fermentation condition together with the selection of suitable microorganisms, health beneficial functional proprieties of tempeh can be obtained. This gives a possibility to manufacture the products of specific (desirable for customers) features. The knowledge about modeling chemical composition, as well as biological activities and health effects of tempeh type products is still insufficient and requires more studies.
6

Host immune response in B-cell lymphomas: friend or foe?

51%
Juszczynski P., Nowak J., Warzocha K.
Archivum Immunologiae et Therapiae Experimentalis
|
2008
|
vol. 56
|
issue 4
245-255
EN
The interaction of B-cell malignancies with the host immune system is a dynamic and bilateral process. Certain lymphomas more commonly arise against a background of autoimmunity or chronic infection. Initiation of these tumors is commonly reliant on antigenic stimulation and/or T-cell help. Apart from its tumor-fueling role, the host immune response plays a critical role in cancer immunosurveillance and immunoediting. The concept of immunoediting holds that the immune system sculpts the tumor's immunogenicity in a dynamic process that involves three essential phases: elimination, equilibrium, and escape. Data obtained by studying gene-targeted animal and human lymphomas that support the critical role of the immune response in the initiation, progression, and immunoediting of lymphoid malignancies are summarized here. A thorough understanding of this interaction will lead to the identification of more rational treatment targets and improved immunotherapies in B-cell lymphomas.
7

Regional distribution of heterozygous 657del5 mutation carriers of the NBS1 gene in Wielkopolska province (Poland)

51%
Ziolkowska I., Mosor M., Nowak J.
Journal of Applied Genetics
|
2006
|
vol. 47
|
issue 3
269-272
EN
The homozygous 657del5 mutation, called Slavic mutation, of the NBS1 gene, causes the Nijmegen Breakage Syndrome (NBS). This syndrome is connected with a high incidence of malignancies in early childhood. A high frequency of NBS heterozygotes was found among patients with melanoma, breast, ovary and prostate cancer. The aim of our research was to determine the frequency of 657del5 mutation of the NBS1 gene in the population of Wielkopolska province. For this purpose, we analysed blood samples from anonymous Guthrie cards. In a group of 2090 newborns from the whole province, we found 16 heterozygous mutation carriers. The frequency of 1/131 is higher than 1/190 reported for populations from other regions in Poland. We observed differential regional distribution of heterozygous 657del5 mutation carriers within the province: among 464 samples from the eastern part of Wielkopolska we found 6 carriers (1/77), in contrast to the southern part without any carrier among 625 samples analysed. The high mean frequency of heterozygous 657del5 mutation (1/131) in Wielkopolska province may be associated with cancer incidence in this region.
8
Content available remote

Parallel Mappings as a Key for Understanding the Bioinorganic Materials

51%
Kuczumow A., Nowak J., Chałas R.
Acta Physica Polonica A
|
2009
|
vol. 115
|
issue 2
482-488
EN
Important bioinorganic objects, both living and fossilized are as a rule characterized by a complex microscopic structure. For biological samples, the cell-like and laminar as well as growth ring structures are among most significant. Moreover, these objects belong to a now widely studied category of biominerals with composite, inorganic-organic structure. Such materials are composed of a limited number of inorganic compounds and several natural organic polymers. This apparently simple composition leads to an abnormal variety of constructions significant from the medical (repairs and implants), natural (ecological effectiveness) and material science (biomimetic synthesis) point of view. The analysis of an image obtained in an optical microscope, optionally in a scanning electron microscope is a topographical reference for further investigations. For the characterization of the distribution of chemical elements and compounds in a material, techniques such as X-ray, electron- or proton microprobes are applied. Essentially, elemental mappings are collected in this stage. The need for the application of an X-ray diffraction microprobe is obvious and our experience indicates on the necessity of using the synchrotron-based devices due to their better spatial resolution and good X-ray intensity. To examine the presence of the organic compounds, the Raman microprobe measurements are good options. They deliver information about the spatial distribution of functional groups and oscillating fragments of molecules. For the comprehensive investigation of bioinorganic material structural and chemical features, we propose the following sequence of methods: optical imaging, elemental mapping, crystallographic mapping, organic mapping and micromechanical mapping. The examples of such an approach are given for: petrified wood, human teeth, and an ammonite shell.
9
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Content available

Composite Zones Produced in Situ in the Ni3Al Phase

45%
Olejnik E., Tokarski T., Janas A., Sikora G., Nowak J.
Archives of Foundry Engineering
|
2013
|
issue 1
10
Publication available in full text mode
Content available

The Production of Material with Ultrafine Grain Structure in Al-Zn Alloy in the Process of Rapid Solidification

45%
Szymaneka M., Boczkal S., Augustyn B., Kapinos D., Nowak J.
Archives of Foundry Engineering
|
2014
|
issue 2
11
Publication available in full text mode
Content available

Producing Ultrafine Grain Structure in AZ91 Magnesium Alloy Cast by Rapid Solidification

45%
Szymanek M., Augustyn B., Kapinos D., Boczkal S., Nowak J.
Archives of Metallurgy and Materials
|
2014
|
issue 1
12

Immune response against genetically modified tumor cells

27%
Nowak J., Januszkiewicz D., Kowalczyk D., Ma, Mazurek J., Laciak M., Malicki J., Murawa P., Wiznerowicz M., Heinrich P. C., Rose-John S.
Biotechnologia
|
1996
|
issue 4
55-67
EN
The provisional conclusion is that 'anti-cancer vaccine' may enhance unspecific and specific anti-tumor immune response in some of the treated malignant melanoma patients.
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