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Human neutrophil peptide 3 could be functionally expressed in Rhodobacter sphaeroides

100%

Nie X., Zhang L., Hu Z., Liu Y., Zhao Z.

2015

vol. 62

issue 2

259-263

Human neutrophil peptides (HNPs) possess high antimicrobial activities against a broad spectrum of microorganisms. Rhodobacter sphaeroides is the best-characterized photosynthetic bacterium and exhibits potential as a novel expression system. Up to date, no literature has been reported regarding expression of HNP3 in Rb. sphaeroides. In the present study, the HNP3 gene fragment was amplified by SOE PCR and ligated into photosynthetic bacteria light-harvesting complex 2 (LH2) expression vector leading to HNP3 fusion protein expression vector. The HNP3 fusion protein was successfully expressed as rapidly evaluated by the LH2 characteristic peaks at ~800 nm and ~850 nm before purification and SDS/PAGE. Subsequently, the HNP3 fusion protein was purified by one-step affinity chromatography, and could be rapidly detected by the color and the spectral absorption at ~800 nm and ~850 nm before SDS/PAGE. Antimicrobial activity assay suggested that the HNP3 fusion protein exhibited high antimicrobial activity towards E. coli. The present study may supply an insight into employing the novel Rb. sphaeroides expression system, exhibiting dramatic advantages over currently used commercial expression system, to heterologously express human neutrophil peptides.

An ab initio simulation of the UV/Visible spectra of substituted chalcones

86%

Xue Y., Mou J., Liu Y., Gong X., Yang Y., An L.

Open Chemistry

2010

vol. 8

issue 4

928-936

The electronic absorption spectra of 29 phenyl-ring substituted chalcones have been investigated with the time-dependent density functional theory (TD-DFT) and polarizable continuum TD-DFT (PCM-TD-DFT). It turns out that the hybrid PBE1PBE functional with the 6-31G basis set provide reliable λmax when the solvent effects are included in the model. Comparisons with experimental values lead to a mean absolute error of 12 nm (0.136 eV). Moreover, the observed substituent effects are reproduced by calculation qualitatively. The λmax of substituted chalcone in phenyl ring A is less sensitive to substitution than that in ring B. The linear correlation of Hammett’s substituent constants (σP) with LUMO energies is better with respect to HOMO energies. The calculation reveals that the maximum absorption band mainly results from the π→π* transition from HOMO to LUMO. The analysis of the electron density plots of frontier molecular orbitals show that most transitions should be of valence excitation nature. [...]

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1 Acta Biochimica Polonica

1 Open Chemistry

1 2015

1 2010

2 Liu Y.

1 An L.

1 Gong X.

1 Hu Z.

1 Mou J.

1 Nie X.

1 Xue Y.

1 Yang Y.

1 Zhang L.

1 Zhao Z.

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Human neutrophil peptide 3 could be functionally expressed in Rhodobacter sphaeroides

An ab initio simulation of the UV/Visible spectra of substituted chalcones