Search results

1

Endocannabinoid system and anticancer properties of cannabinoids

100%

Śledziński P., Nowak A., Zeyland J., Słomski R.

Acta Universitatis Lodziensis. Folia Biologica et Oecologica

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2016

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vol. 12

11-25

PL

Kannabinoidy oddziałują na organizm ludzki wiążąc się z receptorami kannabinoidowymi (CB1 oraz CB2). Dwoma głównymi kannabinoidami roślinnymi są Δ9-tetrahydrokannabinol (THC) i kannabidiol (CBD). THC wiąże się z receptorami CB1 obecnymi w obrębie centralnego układu nerwowego, co powoduje psychoaktywne właściwości marihuany. CBD posiada niskie powinowactwo do receptorów CB1, nie posiada właściwości psychoaktywnych, co sprawia, że jego medyczne zastosowanie może być znacznie szersze. Receptory CB są częścią złożonego mechanizmu zaangażowanego w regulację wielu procesów fizjologicznych – układu endokannabinoidowego. Kannabinoidy znalazły pewne zastosowanie w medycynie paliatywnej, lecz istnieje wiele badań dowodzących ich antynowotworowych właściwości. Agoniści receptorów CB1 powodują akumulację związków z grupy ceramidów w komórkach nowotworowych, stres retikulum endoplazmatycznego i w konsekwencji apoptozę. W efektach wywoływanych przez kannabinoidy posiadając niskie powinowactwo do receptorów CB pośredniczy najprawdopodobniej indukcja produkcji reaktywnych form tlenu. Dotychczasowa wiedza dotycząca przeciwnowotworowych właściwości kannabinoidów opiera się tylko na badaniach przedklinicznych. Istnieje potrzeba przeprowadzania kolejnych badań, które umożliwiłyby oszacowanie rzeczywistego potencjału tych związków.

EN

Cannabinoids impact human body by binding to cannabinoids receptors (CB1 and CB2). The two main phytocannabinoids are Δ9-tetrahydrocannabinol (THC) and cannabidiol (CBD). THC interacts with CB1 receptors occurring in central nervous system and is responsible for psychoactive properties of marijuana. CBD has low affinity to CB1 receptor, has no psychoactive characteristics and its medical applications can be wider. CB receptors are part of a complex machinery involved in regulation of many physiological processes – endocannabinoid system. Cannabinoids have found some applications in palliative medicine, but there are many reports concerning their anticancer affects. Agonists of CB1 receptors stimulate accumulation of ceramides in cancer cells, stress of endoplasmic reticulum (ER stress) and, in turn, apoptosis. Effects of cannabinoids showing low affinity to CB receptors is mediated probably by induction of reactive oxygen species production. Knowledge of antitumor activity of cannabinoids is still based only on preclinical studies and there is a necessity to conduct more experiments to assess the real potential of these compounds.

2

Biotechnological conversion of glycerol from biofuels to 1,3-propanediol using Escherichia coli

100%

Przystałowska H., Lipiński D., Słomski R.

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2015

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vol. 62

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issue 1

23-34

EN

In the face of shortage of fossil fuel supplies and climate warming triggered by excessive carbon dioxide emission, alternative resources for chemical industry have gained considerable attention. Renewable resources and their derivatives are of particular interest. Glycerol, which constitutes one of the by-products during biodiesel production, is such a substrate. Thus, generated excess glycerol may become an environmental problem, since it cannot be disposed of in the environment. The most promising products obtained from glycerol are polyols, including 1,3-propanediol, an important substrate in the production of synthetic materials, e.g. polyurethanes, unsaturated polyesters, and epoxy resins. Glycerol can be used as a carbon and energy source for microbial growth in industrial microbiology to produce 1,3-propanediol. This paper is a review of metabolic pathways of native producers and E. coli with the acquired ability to produce the diol via genetic manipulations. Culture conditions during 1,3-PDO production and genetic modifications of E. coli used in order to increase efficiency of glycerol bioconversion are also described in this paper.

3

Application of non-radioactive methods of DNA detection in analisys of human genetic disorders

100%

Słomski R., Reiss J., Jungerman M.

Acta Biochimica Polonica

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1989

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vol. 36

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issue 3-4

311-321

4

Understanding cannabinoid receptors: structure and function

88%

Andrzejewska A., Staszak K., Kaczmarek-Ryś M., Słomski R., Hryhorowicz S.

Acta Universitatis Lodziensis. Folia Biologica et Oecologica

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2018

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vol. 14

1-13

PL

Układ endokannabinoidowy (ang. endocannabinoid stystem-ECS) składa się z receptorów kannabinoidowych CB1 i CB2, agonistów egzogennych i endogennych: kannabinoidów i endokannabinoidów oraz enzymów regulujące syntezę i degradację endogennych ligandów tego układu. Jest systemem biorącym udział w wielu procesach fizjologicznych organizmu. Odgrywa on istotną rolę w prawidłowym funkcjonowaniu centralnego i autonomicznego układu nerwowego. Układ endokannabinoidowy reguluje również m.in. gospodarkę energetyczną, aktywność motoryczną, układ endokrynny oraz układ hormonalny poprzez regulacje powiązań neurohormonalnych i neuroimmunologicznych. Wiele jego wspomnianych funkcji związanych jest z receptorami sprzężonymi z białkami G, takimi jak receptory CB1, CB2 czy GPR55. Co raz więcej mówi się również o znaczącej roli receptorów przejściowego potencjału TRPs (ang. transient receptor potential), które nie tylko pośredniczą w odczuwaniu bólu, ale także są odpowiedzialne za wspomaganie stanu zapalnego poprzez wydzielanie pro-zapalnych neuropeptydów. W niniejszej pracy przeglądowej postanowiliśmy krótko scharakteryzować oraz opisać rolę i działanie receptorów kannabinoidowych CB1 i CB2, receptora sprzężonego z białkiem G (GPR55) i receptora przejściowego potencjału waniloidowego (TRPV1).

EN

The endocannabinoid system (ECS) consists of the endocannabinoids, cannabinoid receptors and the enzymes that synthesize and degrade endocannabinoids. The whole EC system plays an important role in the proper functioning of the central and autonomic nervous system. ECS is involved in the regulation of the body energy and in the functioning of the endocrine system. It can affect on the regulation of emotional states, motoric movement, operations of the endocrine, immune and digestive system. Many of the effects of cannabinoids are mediated by G coupled –protein receptors: CB1, CB2 and GPR55 but also of transient receptor potential channels (TRPs) which not only induce the sensation of pain but also support inflammation via secretion of pro-inflammatory neuropeptides. In this review work we briefly summarize the role and action of cannabinoid receptors CB1 and CB2, protein-coupled receptor 55 (GPR55) and transient receptor potential vanilloid 1 (TRPV1).

5

Haplotype analysis of phenylalanine hydroxylase alleles in Polish families with phenyloketonuria

88%

Jaruzelska J., Borski K., Riess O., Blin N., Słomski R.

Acta Biochimica Polonica

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1989

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vol. 36

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issue 3-4

323-332

6

Maximal oxygen uptake is associated with allele -202 A of insulin-like growth factor binding protein-3 (IGFBP3) promoter polymorphism and (CA)n tandem repeats of insulin-like growth factor IGF1 in Caucasians from Poland

88%

Gronek P., Holdys J., Kryściak J., Wieliński D., Słomski R.

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2014

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vol. 61

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issue 2

245-252

EN

Physical fitness is a trait determined by multiple genes, and its genetic basis is modified by numerous environmental factors. The present study examines the effects of the (CA)n tandem repeats polymorphism in IGFI gene and SNP Alw21I restriction site -202 A>C polymorphism in IGF1BP3 on VO2max - a physiological index of aerobic capacity of high heritability. The study sample consisted of 239 (154 male and 85 female) students of the University School of Physical Education in Poznań and athletes practicing various sports, including members of the Polish national team. An association was found between -202 A/C polymorphism of IGFBP3 gene with VO2max in men. Higher VO2max values were attained by men with CC genotype, especially male athletes practicing endurance sports and sports featuring energy metabolism of aerobic/anaerobic character. A statistically significant influence of allele 188 and genotype 188/188 of tandem repeats (CA)n polymorphism of IGF1 gene on VO2max was found in women. Also, lower values of maximal oxygen uptake were noted in individuals with allele 186 or genotype 186/186, and higher VO2max values in athletes with allele 194.

7

Increased Frequency of Ace Gene DD Genotype in Normotensive Male Smokers with Abdominal Aortic Aneurysm

88%

Waliszewski K., Słomski R., Oszkinis G., Majewski W., Pawlaczyk K.

Polish Journal of Surgery

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2007

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vol. 79

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issue 5

368-374

EN

The aim of the study was to assess whether the association between abdominal aortic aneurisms (AAAs) and the angiotensin-converting enzyme (ACE) DD genotype is changed in male smokers.Material and methods. The ACE I/D polymorphism was examined by PCR in 112 subjects with AAA and in 50 control subjects. The frequency of ACE genotypes (II, ID and DD) in the patient group was assessed in subgroups divided on the basis of blood pressure, smoking and sex.Results. A significant increase in the frequency of ACE DD genotypes has been found in all AAA patients studied (odds ratio OR=2.6; range 1.3-5.6, p <0.0002) when compared to the remaining ACE genotypes (ID and II) and the control group. The respective values were higher when all 74 male smokers with AAA were assessed (OR=3.6; p <0.006). No increase in ACE DD genotype frequency was seen in all females (38) and male nonsmokers when compared to the respective control group (n=16) (OR=1.4; p <0.75).In the group of normotensive AAA patients, the proportion of ACE DD genotype in relation to the remaining ACE genotypes (ID and II) was found to be higher than in the general AAA patient population (odds ratio OR=6.14; range 2.5-14.9, p <0.0001). In 32 normotensive male smokers with AAA, the respective values were higher (OR=8.3).Conclusions. An increased frequency of the ACE DD genotype in normotensive male smokers may account in large part for the increase in DD genotype occurrence noted in all AAA patients.

8

Isolation of low-molecular albumins of 2S fraction from soybean (Glycine max (L.) Merrill)

88%

Galbas M., Porzucek F., Woźniak A., Słomski R., Selwet M.

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2013

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vol. 60

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issue 1

107-110

EN

Numerous studies have shown that consumption of soybean products decrease the risk of cancers in humans. Experiments at the molecular level have demonstrated that in most cases proteins and peptides are responsible for the anticancer properties of soybeen. Special attention should be paid to lunasin - a peptide described for the first time 16 years ago. Due to its structure it causes i.a., inhibition of cancer cell proliferation. A novel procedure for the isolation and purification of low-molecular-mass 2S soybean albumin protein is described in the present paper. A fraction of four peptides one of them corresponding to molecular mass and isoelectric point characteristic for lunasin. The obtained peptides decreased on the rate of HeLa cell proliferation.

9

Partial purification, characterization and cell-free translation of mRNAs coding for H-2^d antigens

87%

Witt M., Słomski R., Kurpisz M.

Acta Biochimica Polonica

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1982

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vol. 29

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issue 3-4

311-319

10

Association between estrogen receptor alpha gene polymorphisms and bone mineral density in Polish female patients with Graves' disease

76%

Ignaszak-Szczepaniak M., Horst-Sikorska W., Dytfeld J., Gowin E., Słomski R., Stajgis M.

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2011

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vol. 58

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issue 1

101-109

EN

Graves' (GD) hyperthyroidism leads to reduced bone mineral density (BMD) accompanied by accelerated bone turnover. Ample studies have identified association between estrogen receptor (ESR1) gene polymorphism and decreased BMD and osteoporosis. In contrast, number of publications that link ESR1, BMD and Graves' disease is limited. The purpose of this study was to identify the association between ESR1 polymorphisms and BMD in premenopausal women with GD and to determine whether ESR1 polymorphic variants can predispose to GD. The study included 75 women aged 23-46 years with GD and 163 healthy controls. BMD was measured at lumbar spine and femoral neck. We investigated two SNPs in the ESR1 gene and analyzed genetic variants in the form of haplotypes reconstructed by statistical method. Three out of four possible haplotypes of the PvuII and XbaI restriction fragment length polymorphisms were found in GD patients: px (55.3 %), PX (33.3 %) and Px (11.4 %). Women homozygous for xx of XbaI and for pp of PvuII had the lowest BMD at lumbar spine. Moreover, the px haplotype predisposed to reduced lumbar BMD. No associations were observed for femoral neck BMD. No statistically significant relationship were found between ESR1 polymorphisms or their haplotypes and GD. These results indicate that the PvuII and the XbaI polymorphisms of ESR1 gene are associated with bone mineral density in premenopausal women with GD and may help to estimate the risk of bone loss particularly at lumbar spine. However, none of the ESR1 gene alleles predict the risk of GD in Polish female patients.

11

Virological aspects of non-human primates or swine-to human xenotransplantation

76%

Mazurkiewicz N., Nowak A., Hryhorowicz M., Zeyland J., Lipiński D., Słomski R.

Acta Universitatis Lodziensis. Folia Biologica et Oecologica

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2018

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vol. 14

47-54

PL

Istnieje wiele chorób człowieka, które mogą doprowadzić do niewydolności narządów. Konsekwencją często jest konieczność przeprowadzenia przeszczepu. Liczba wykonywanych operacji jest bardzo niska ze względu na niedobór narządów do transplantacji. W konsekwencji liczba osób oczekujących na przeszczep ciągle rośnie. Wyjściem z tej sytuacji może być ksenotransplantacja. Pojęcie ksenotransplantacja pochodzi od greckiego xenos oznaczającego obcy, inny. Jest to każdy zabieg polegający na transplantacji, implantacji lub infuzji biorcy – człowiekowi – komórek, tkanek lub organów odzwierzęcych, również płynów ustrojowych, komórek, tkanek, narządów człowieka (lub ich fragmentów), które miały kontakt ex vivo z komórkami, tkankami lub organami zwierzęcymi. Jedną z przeszkód w przeszczepach ksenogenicznych jest zagrożenie przeniesienia patogenów zwierzęcych i zainfekowanie organizmu człowieka. Wirusami, które stanowią zagrożenie w przeszczepach, w układzie małpy naczelne-człowiek to między innymi: ludzki wirus upośledzenia odporności HIV (z ang. human immunodeficiency virus) i wirus Marburg, które zostały opisane w niniejszej pracy. Ponadto przedstawiono wirusy stanowiące problem w transplantacjach w układzie świnia-człowiek, czyli: endogennego retrowirusa PERV (z ang. porcine endogenous retrovirus), wirusa cytomegalii świni PCMV (z ang. porcine cytomegalovirus), wirusa limfotroficznego świni PLHV (z ang. porcine lymphotropic herpesvirus) oraz wirusa zapalenia wątroby typu E - HEV (z ang. hepatitis E virus). Niniejszy przegląd literatury stanowi najnowszy stan wiedzy na temat mikrobiologicznego bezpieczeństwa ksenotransplantacji.

EN

There are a number of human diseases, which can lead to organ failure. The consequence is often the need for a transplant. The number of performed operations is very low due to the shortage of organs for transplantation. As a consequence, the number of people waiting for transplant is still growing. The solution to this situation may be xenotransplantation. Xenotransplantation word comes from the Greek xenos meaning stranger, the other. It is defined as any procedure that involves the transplantation, implantation or infusion of tissues or zoonotic organs into a human recipient, but also human body fluids, cells, tissues, organs (or fragments) that have ex vivo contact with zoonotic cells, tissues or organs. One of the obstacles of the xenograft transplantation is the risk of animal pathogens transmission to the humans. Viruses that pose risk in the non-human primates-to-human xenotransplantation includes: the human immunodeficiency virus - HIV and the Marburg virus described in this paper. In addition viruses, which is a problem in pig-to-human xenotransplantation have also been described, including: porcine endogenous retrovirus - PERV, porcine cytomegalovirus - PCMV, porcine lymphotropic herpesvirus - PLHV and hepatitis E virus - E - HEV. This review of literature is the latest knowledge of the microbiological safety of xenotransplantation.

12

Development of the UHPLC-DAD method for simultaneous determination of four active pharmacological ingredients in cannabinoid plant material

76%

Zalewski P., Krause A., Milewski M., Wielgus K., Szalata M., Słomski R., Piontek J. C.

Acta Poloniae Pharmaceutica - Drug Research

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2018

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vol. 75

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issue 4

885-890

EN

A stability-indicating ultra-high-performance liquid chromatography (UHPLC) with DAD detector was developed and validated for the simultaneously determination of Δ9-tetahydrocannabitol (THC), cannabidiol (CBD), Δ9-tetahydrocannabinolic acid (THC-COOH) and cannabidiolic acid (CBD-COOH) in extracts of Cannabis sativa L.. The separation was achieved on a Kinetex, C18, 100 x 2.1 mm, 2.6 µm. The gradient separation was started with mixture of methanol and 12mM ammonium acetate in ratio 75:25 (%/%). The methanol was increased to 100% from 0.0 min to 10.0 min, then maintained 100% for the next 1.0 min, then decreased to 75% from 11.0 min to 15.0 min and held at 75% from 15.0 min to 20.0 min. The injection volume was 5.0 µL and the wavelength of detection was controlled at 228 nm.

13

1,3-Propanediol production by Escherichia coli using genes from Citrobacter freundii atcc 8090

76%

Przystałowska H., Zeyland J., Kośmider A., Szalata M., Słomski R., Lipiński D.

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2015

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vol. 62

|

issue 3

589-597

EN

Compared with chemical synthesis, fermentation has the advantage of mass production at low cost, and has been used in the production of various industrial chemicals. As a valuable organic compound, 1,3-propanediol (1,3-PDO) has numerous applications in the production of polymers, lubricants, cosmetics and medicines. Here, conversion of glycerol (a renewable substrate and waste from biodiesel production) to 1,3-PDO by E. coli bacterial strain carrying altered glycerol metabolic pathway was investigated. Two gene constructs containing the 1,3-PDO operon from Citrobacter freundii (pCF1 and pCF2) were used to transform the bacteria. The pCF1 gene expression construct contained dhaBCE genes encoding the three subunits of glycerol dehydratase, dhaF encoding the large subunit of the glycerol dehydratase reactivation factor and dhaG encoding the small subunit of the glycerol dehydratase reactivating factor. The pCF2 gene expression construct contained the dhaT gene encoding the 1,3-propanediol dehydrogenase. Expression of the genes cloned in the above constructs was under regulation of the T7lac promoter. RT-PCR, SDS-PAGE analyses and functional tests confirmed that 1,3-PDO synthesis pathway genes were expressed at the RNA and protein levels, and worked flawlessly in the heterologous host. In a batch flask culture, in a short time applied just to identify the 1,3-PDO in a preliminary study, the recombinant E. coli bacteria produced 1.53 g/L of 1,3-PDO, using 21.2 g/L of glycerol in 72 h. In the Sartorius Biostat B Plus reactor, they produced 11.7 g/L of 1,3-PDO using 24.2 g/L of glycerol, attaining an efficiency of 0.58 [mol1,3-PDO/molglycerol].

14

DNA Bank for Polish Patients with a Predisposition for Intestinal Polyposis

76%

Pławski A., Podralska M., Cichy W., Drews M., Krokowicz P., Słomski R.

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issue 10

465-473

EN

Intestinal polyposis syndromes include a group of diseases conditioned by the occurrence of hereditary mutations. The current paper presents a collection of DNA samples derived from persons from families with a diagnosed adenomatous polyposes which comprise: familial polyposis coli together with its recessive form, Turcot's syndrome, inherited mixed polyposis as well as persons with recognised hamartomatous polyposes: juvenile polyposis, Peutz-Jeghers syndrome, Cowden syndrome and Proteus syndrome.The aim of the study was to present current achievements associated with the establishment of the DNA Bank for intestinal polyposis.Material and methods. Investigations were conducted on DNA isolated from cells of the peripheral blood. The search for mutations in APC, MUTYH, PTEN, BMPR1A, SMAD4 and STK11 genes preconditioning the occurrence of individual diseases was performed employing PCR-SSCP, PCR-HD, DHPLC as well as RFLP techniques and DNA sequencing.Results. At the present time, the DNA Bank comprises the total of 1097 DNA samples derived from 449 families with intestinal polyposis of which 945 samples come from persons in whose families Familial Adenomatous Polyposis (FAP) occurred. In addition, the collected data also contain material for analyses derived from 25 families with Peutz-Jeghers syndrome and 20 families with juvenile polyposis as well as single cases with the Cowden syndrome, Proteus syndrome and desmoid tumors. The performed molecular investigations allowed identification of mutations ranging from 44 to 50%.Conclusions. With regard to the quantity of the material collected for analyses and the efficacy level of the employed molecular methods, the obtained results are in keeping with the results found in the literature from the field of genetics and medicine and do not differ from world standards. The collection of data and materials for investigations in the case of rare diseases allows qualitative, organisational and economic optimisation of the performed investigations.

15

Cytotoxicity of thermo-responsive polymeric nanoparticles based on N-isopropylacrylamide for potential application as a bioscaffold

64%

Deptuła T., Warowicka A., Woźniak A., Grzeszkowiak M., Jarzębski M., Bednarowicz M., Patkowski A., Słomski R.

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2015

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vol. 62

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issue 2

311-316

EN

Polymeric nanoparticles based on poly-N-isopropylacrylamide (pNiPAM NPs) and their bio-medical applications have been widely investigated in recent years. These tunable nanoparticles are considered to be great candidates for drug delivery systems, biosensors and bioanalytical devices. Thus, the biocompatibility and toxicity of these nanoparticles is clearly a crucial issue. In this work, the cytotoxicity of thermo-responsive pNiPAM nanoparticles was studied, followed by a detailed analysis of the NPs morphology in growing cell cultures and their 3D structure. Cytotoxic examination was conducted for two cell cultures - HeLa (cervical cancer cell line) and HeK293 (human embryonic kidney cell line), employing MTT (3-4, 5-dimethylthiazol-2-yl-2, 5-diphenyltetrazolium bromide) assay and viability tests. We used Cryo-SEM (scanning electron microscopy) and fluorescence microscopy (IN Cell Analyzer) in order to investigate the morphological structure of the polymer network. We show that pNiPAM nanoparticles do not exhibit any cytotoxicity effects on the investigated cell lines. Additionally, we report that the pNiPAM nanoparticle based scaffold promotes cell growth.

16

Some aspects of structure of murine H-2^d antigens

62%

Witt M., Słomski R.

Acta Biochimica Polonica

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1983

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vol. 30

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issue 3-4

303-310

Refine search results

Endocannabinoid system and anticancer properties of cannabinoids

Biotechnological conversion of glycerol from biofuels to 1,3-propanediol using Escherichia coli

Application of non-radioactive methods of DNA detection in analisys of human genetic disorders

Understanding cannabinoid receptors: structure and function

Haplotype analysis of phenylalanine hydroxylase alleles in Polish families with phenyloketonuria

Maximal oxygen uptake is associated with allele -202 A of insulin-like growth factor binding protein-3 (IGFBP3) promoter polymorphism and (CA)n tandem repeats of insulin-like growth factor IGF1 in Caucasians from Poland

Increased Frequency of Ace Gene DD Genotype in Normotensive Male Smokers with Abdominal Aortic Aneurysm

Isolation of low-molecular albumins of 2S fraction from soybean (Glycine max (L.) Merrill)

Partial purification, characterization and cell-free translation of mRNAs coding for H-2^d antigens

Association between estrogen receptor alpha gene polymorphisms and bone mineral density in Polish female patients with Graves' disease

Virological aspects of non-human primates or swine-to human xenotransplantation

Development of the UHPLC-DAD method for simultaneous determination of four active pharmacological ingredients in cannabinoid plant material

1,3-Propanediol production by Escherichia coli using genes from Citrobacter freundii atcc 8090

DNA Bank for Polish Patients with a Predisposition for Intestinal Polyposis

Cytotoxicity of thermo-responsive polymeric nanoparticles based on N-isopropylacrylamide for potential application as a bioscaffold

Some aspects of structure of murine H-2^d antigens