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Application of electrophoretic methods for detection of protein-porphyrin complexes.

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Kowalska A., Kwiek P., Miłosz E., Gondek G., Romiszewska A., Graczyk A., Podhajska A. J.
|
2003
|
vol. 50
|
issue 4
1155-1163
EN
Simple methods for detection and isolation of protein-porphyrin complexes were elaborated in our laboratory. They are based on the separation of protein-porphyrin complexes in native polyacrylamide gel and measurement of their fluorescence, with the use of two detection systems: the commercially available Gel DocTM 2000 system, and a system specially designed for the purpose of these investigations, concerning protein-porphyrin interactions. The fluorescent complexes can be electro-transferred from the gel onto PVDF membrane, eluted and analyzed in order to identify the protein interacting with porphyrins.
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