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A Study on Cultivation of Indigenous Mushrooms in South Eastern Nigeria

100%

Okigbo R. N., Ezebo R. O., Nwatu C. M., Omumuabuike J. N., Esimai G. B.

World News of Natural Sciences

2021

vol. 34

154-164

Studies were carried out to investigate cultivation of some indigenous edible mushrooms in Anambra State. The most suitable substrates for cultivation of Pleurotus tuberregium and Volvariella volvacea were also investigated. Spawn of V. volvacea was obtained from the Department of Life Science, University of Benin, Edo State and sclerotia of P. Tuberregium purchased from Eke Awka Market, Anambra State were used for this study. Plantain leaves and oil palm bunch wastes were substrates employed for V. volvacea whereas topsoil was used for P. tuberregium cultivation. The methods used were site preparation, substrate preparation, soil preparation, sclerotia preparation, cultivation of sclerotia, irrigation, spawn cultivation and harvesting of mushrooms. Means were analyzed statistically using (ANOVA) to test for significance. Means were separated using Duncan’s Multiple Range Tests (DMRT).The highest number of fruit bodies of V. volvacea, 312.68 was obtained from plantain leaves on day 2 after primordial emergence whereas the least, 21.00 was recorded from oil palm bunch wastes on days 6 and 14, respectively. The highest number of P. tuberregium, 61.58 was recorded on day 12 after primordial emergence while the least, 20.49 was recorded on days 7, 13 and 14, respectively. All the three substrates utilized for cultivation of mushrooms in this study supported their growth and development. Plantain leaves supported fast colonization and produced high yield fruit bodies of V. volvacea. The ability to use agricultural wastes for cultivation of mushrooms will boost food production for ever increasing population.

Effect of Protein Extract of Mangifera indica Pollen on the Erythrocytes (Red Blood Cell) of Albino Rats

86%

Ezebo R. O., Omumuabuike J. N., Esimai B. G., Ilouno E. E., Obieze O. U., Obienyem J. N., Uba C. A.

World News of Natural Sciences

2021

vol. 36

114-124

This study was carried out to show the effect of the protein extract of Mangifera indica on the red blood cell (erythrocytes) of albino rats. The Mangifera protein was extracted using 500ml of 0.02m (20mM) phosphate- buffered saline (PBS), at pH 7.4, in a large beaker (stirred with a magnetic stirrer for 3 hours at room temperature). The crude extract was saturated to 60% by adding solid ammonium sulphate under constant gentle attiring, and then stored in a refrigerator for 6 hours. Sixteen male albino rats obtained from animal house in Faculty of Biological Science, University of Nigeria Nsukka was used for the study. They were divided into two groups of eight. One group was labeled the experimental group and the other control. The extract was administered to the experimental rats intra nasally for a period of seven (7) days. Data were expressed as mean ± standard error. Means were separated using Duncan’s New Multiple Range Test (DNMRT). Blood samples were collected via the orbital plexus of rats to determine the effect of the extract on red blood cell (erythrocytes). The present study demonstrated that the extracted pollen of Mangifera indica had no allergic effect on ratsand so would need to be further investigated.

Phytochemical Screening and Antimicrobial Activity of Ethanol and Methanol Extracts of Lantana camara Leaf

73%

Ezebo R. O., Okonkwo C. C., Ozoh C. N., Nwankwo C. A., Nwafor E. C., Esimai B. G., Achonye C. C., Obienyem J. N.

World News of Natural Sciences

2021

vol. 37

151-163

This study investigated the phytochemical composition and antimicrobial activities of ethanol and methanol leaf extracts of Lantana camara Linn against some clinical pathogens. The ethanol and methanol extracts were obtained by soaking each of the powdered leaf in each solvent. The soaked powdered leaf was allowed to stand for four days at room temperature and later filtered using Whatman filter paper. The filtrate was further concentrated using rotary evaporator and then freeze-dried. The minimum inhibitory concentration (MIC) of the ethanol and methanol leaf extracts was carried out using agar well diffusion method. The phytochemical analysis was done using standard techniques. Data were analysed using Analysis of Variance (ANOVA) to test for significance. Means were separated using Duncan’s Multiple Range Test (DMRT). The results of the antimicrobial activity revealed that V. cholerae was the most susceptible while E. coli was the most resistant to plant extracts. The phytochemicals present in the plant leaf had antimicrobial properties and may serve as a good substitute for resistant human pathogens.

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3 World News of Natural Sciences

3 2021

3 Ezebo R. O.

2 Esimai B. G.

2 Obienyem J. N.

2 Omumuabuike J. N.

1 Achonye C. C.

1 Esimai G. B.

1 Ilouno E. E.

1 Nwafor E. C.

1 Nwankwo C. A.

1 Nwatu C. M.

1 Obieze O. U.

1 Okigbo R. N.

1 Okonkwo C. C.

1 Ozoh C. N.

1 Uba C. A.

Search results

A Study on Cultivation of Indigenous Mushrooms in South Eastern Nigeria

Effect of Protein Extract of Mangifera indica Pollen on the Erythrocytes (Red Blood Cell) of Albino Rats

Phytochemical Screening and Antimicrobial Activity of Ethanol and Methanol Extracts of Lantana camara Leaf