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1

Preparation of pBIN19-PGIP-based genetic construct for modification of strawberry genome to obtain genotypes resistant to grey mould

100%
Korbin M., Majka J., Gruchala A., Zurawicz E.
Biotechnologia
|
2004
|
issue 2
22-28
EN
Polygalacturonase inhibiting protein (PGIP) cDNA was isolated using RT-PCR from fruit of apple 'Golden Delicious' which was previously wounded and inoculated with Botrytis cinerea. The sequence of the cDNA showed high level of homology with the published sequence of PGIP-cDNA from apple and pear (99,7 and 97,3%) and low similarity with the sequence of cDNA from tomato (51,5%). The obtained cDNA was cloned and used for the binary construct creation. The introduction of the pBIN19-PGIP construct into strawberry plant genome seems to be a promising strategy to obtain a genotype resistant to B. cinerea, causal agent of grey mould ? the most economically important fungal disease of this species.
2

Application of molecular markers in evaluation of selected apple genotypes for four apple scab resistance genes

100%
Korbin M., Keller-Przybylkowicz S., Zurawicz E.
Biotechnologia
|
2008
|
issue 2
153-161
EN
Breeding for scab resistant apple cultivars by pyramiding several genes in the same genetic background is a promising way to control this severe disease caused by Venturia inaequalis. To achieve this goal, molecular potential of expected parental forms should be determined. Screening of 24 genotypes used in apple breeding program for the presence of regions, linked to the resistance to apple scab with DNA markers flanking regions of four major genes Vf, Vbj, Vr and Vm, was conducted in the Research Institute of Pomology and Floriculture. All investigated resistance (R) regions were identified in evaluated population and three clusters representing the group of the genotypes without or with single tested R-gene and two groups of the genotypes, being the parallel donors of 3-4 R-genes, were generated.
3

Evaluation of genetic variation of selected strawberry (Fragaria x ananassa Duch.) cultivars using ISSR technique

100%
Kuras A., Korbin M., Zurawicz E.
Biotechnologia
|
2004
|
issue 2
73-78
EN
Precise evaluation of the parental forms? genetic variation is the base of rational, modern breeding. DNA patterns obtained in ISSR (inter simple sequence repeat) reactions for 24 strawberry cultivars, frequently used in the crossing programme at the Research Institute of Pomology and Floriculture, were analysed. Diversifying genotypes, polymorphic DNA products were observed in reactions with 54 out of 90 tested primers (806-896, #9 UBC). Six primers (811-814, 824, 840) generating 44 polymorphic bands with length from 270 to 2030 bp allowed to diversify all investigated cultivars. The DNA profiles characterising each of genotypes contained 10-19 polymorphic ISSR products.
4

Study on an embryo culture of black currant hybrids obtained as a result of distant hybridisation

100%
Golis A., Pluta S., Zurawicz E.
Biotechnologia
|
2001
|
issue 2
66-71
EN
The subjects of this study were embryos obtained from 22 crossing combinations in the course of distant hybridisation. No embryos were obtained from the following three interspecific hybridisations: Siewka K1 x Josta, Siewka K1 x Czereszniewa and Captivator x Josta. Seeds obtained from these combinations did not hold endosperm, but yielded watered liquid. In the remaining hybridisations, a high percentages of visible embryos increased considerably, especially at the torpedo-shaped stage. An addition of kinetin (1 mg/l) to the media had a better impact on plant regeneration from embryos than the addition of BA (0,5 mg/l). Dependending on the study, up to 83,7% of exposed embryos in the media started to grow and develop under the influence of kinetin.
5

Analysis of plum (Prunus domestica L.) DNA polymorphism using RAPD - PCR technique

100%
Lisek A., Korbin M., Zurawicz E., Rozpara E.
Biotechnologia
|
2004
|
issue 2
76-72
EN
Precise identification of plum cultivars is desired in breeding programme of the species as well as in orchard practise. The aim of the presented studies was the determination of 19 plum cultivars Prunus domestica L. diversity. RAPD-PCR technique with 77 primers (Operon Technologies) from kits OPB, OPG, OPT and OPU was used for the analysis. Fifty-five polymorphic fragments DNA (600-2700 bp) were obtained in reactions with 33 primers. The highest number of polymorphic fragments (3-5) was observed in reactions with primers OPB 07, OPB 18, OPG 09, OPG 10 and OPT 14. The reactions with OPB 07, OPB 18 and OPG 09 allowed to diversify 15 cultivars, except 'Wegierka Zwykla' and clones 'Promis', 'Tolar', 'Nectavit'.
6

Genetic and environmental correlation and path coefficient analysis of fruit yield per bush and other traits in black currants (Ribes nigrum L.)

88%
Madry W., Pluta P. S., Pluta S., Zurawicz Z. E., Zurawicz E.
Journal of Applied Genetics
|
2000
|
vol. 41
|
issue 4
221-236
EN
Twenty-five F1 hybrid families of black currants (Ribes nigrum L.) originating from diallel crosses of five cultivars were assessed. Phenotypic correlation coefficient between full-sib family means (rFS), genetic correlation coefficient between full-sib family expected values (rG) and the environmental correlation were estimated on the basis of two years of observations for 12 quantitative characteristics studied. Evident repeatability of correlations in both years was proved. A considerable consistence between the values of genetic and phenotypic correlation coefficients was also observed. Coefficients of phenotypic and environmental correlation were significant for many pairs of traits. Most of the significant environmental correlation coefficients were found for the same pairs of characteristics for which phenotypic correlation coefficients were significant. This shows that genetic and environmental factors affected similar traits. Fruit yield expressed the largest number of significant correlations with other traits. Path coefficient analysis indicated that plant and berry size were the main factors influencing directly fruit yield in both years. It was proved that the number and length of one-year-old shoots via bush size had very large indirect effects on fruit yield per plant.
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