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EN
The paper presents a study on the microbiological activity and the quality of organic matter of a Haplic Luvisol soil amended with selected organic and mineral materials (manure, clay, flotation lime, aluminium and iron oxides and a bark-keratin-urea preparation), during a three-year field experiment. In the soil under analysis (pH in 1M KCl pHKCl - 4.46, organic carbon Corg. - 0.465%) the following parameters of microbiological activity were assayed: numbers of microorganisms, activity of dehydrogenases, and respiration. In addition, the quality of organic matter was analysed through the determination of its susceptibility to chemical (biological) oxidation (oxidable carbon and non oxidable carbon). The components applied to the soil had a varied effect on the studied parameters of microbiological activity during the whole period of the study. The application of manure and clay caused an increase in the numbers of bacteria, in the activity of dehydrogenases, and in the intensity of the process of soil respiration. Soil respiration was stimulated also by the application of manure and clay in combination with Fe2O3 at the dose of 6 kg. Moreover, a decrease in the number of bacteria in the soil was noted after the combined application of manure, clay and Fe2O3 6 kg. The materials applied in the study caused a variation in the susceptibility of organic matter of Haplic Luvisol soil to oxidation. Soils amended with manure and clay in combination with calcium, aluminium, iron, were characterised by limited susceptibility to organic matter oxidation, while the bark-keratin-urea preparation applied caused a considerable improvement of that susceptibility
PL
W niniejszej pracy badano aktywność mikrobiologiczną oraz jakość substancji organicznej gleby płowej wzbogaconej wybranymi materiałami organicznymi i mineralnymi (obornik, ił, wapno poflotacyjne, tlenek glinu i żelaza oraz preparat koro-keratyno-mocznikowy) podczas trzyletniego doświadczenia polowego. W analizowanej glebie (pHKCl - 4,46; Corg. 0,465%) określano następujące parametry aktywności mikrobiologicznej: liczebność drobnoustrojów, aktywność dehydrogenaz oraz oddychanie. Ponadto analizowano jakość substancji organicznej poprzez określenie jej podatności na chemiczne (biologiczne) utlenianie. Wprowadzone do gleby komponenty w różnym stopniu wpływały na badane parametry aktywności mikrobiologicznej w trakcie całego okresu badań. Zastosowanie obornika i iłu skutkowało wzrostem liczby bakterii, aktywności dehydrogenaz oraz nasileniem procesu oddychania. Oddychanie gleby było stymulowane również przez dodatek obornika i iłu (łącznie z Fe2O3 6 kg). Ponadto odnotowano spadek liczby bakterii w glebie po dodaniu obornika, iłu (łącznie z Fe2O3 6 kg). Zastosowane w doświadczeniu materiały wpłynęły na zróżnicowanie podatności na utlenianie substancji organicznej gleby płowej. Gleby wzbogacone obornikiem, iłem w połączeniu z wapnem, glinem, żelazem charakteryzowały się ograniczoną podatnością substancji organicznej na utlenianie, zaś zastosowany preparat koro-keratyno-mocznikowy przyczynił się do wzrostu tej podatności.
EN
The study was conducted on an experiment established in the area of the former Sulphur Mine “Jeziorko.” The remediation was applied to a soil-less formation with particle size distribution of weakly loamy sand, strongly acidified and with bad sorptive properties (Corg. - 2.0 g kg−1; pHKCl - 4.3; T - 7.0 cmol(+) kg−1). In the particular treatments of the experiment the following were applied to the soil-less formation: flotation lime and NPK; lime and sewage sludge; sewage sludge; mineral wool (5 cm 50 cm−1), lime and NPK; mineral wool (5 cm 50 cm−1), lime and sewage sludge; mineral wool (500 m3 ha−1), lime and NPK; mineral wool (500 m3 ha−1), lime and sewage sludge. Plots prepared in that manner were then sown with a mix of grasses. The control was the soil with no amendments. The analyses of the soil material comprised assays of the numbers of particular groups of bacteria and fungi, and of their biochemical and enzymatic activities. The study revealed that all the wastes applied for the remediation caused an increase in the numbers of the bacterial groups studied (copiotrophic, oligotrophic, cellulolytic, lipolytic), as well as in the respiration activity and rate of mineralisation of cellulose. That effect was the most pronounced in the case of sewage sludge. In treatments in which sewage sludge was applied, an increase was also observed in the numbers of the studied fungi (fungi on Martin medium, cellulolytic fungi, lipolytic fungi) and in lipase activity. Whereas, the application of the remaining wastes resulted in a slight decrease in the numbers of the fungal groups under analysis. Comparing the mean annual values of the analysed biological, physical, chemical and physicochemical properties it was found that the biological properties were as sensitive, and in the case of certain tests (numbers of cellulolytic and lipolytic bacteria, rate of cellulose mineralisation) even more sensitive indicators of positive changes taking place in the remediated soil.
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