Opisano metodą otrzymywania jąder komórkowych i otoczek jądrowych z tkanki łożyska ludzkiego. Preparaty jąder komórkowych zawierały: 56,4% białka, 37% DNA 5,02% fosfolipidów i 1,42% RNA.
Summary The data concerning the structure of the mitochondrial desmolase complex, which converts cholesterol to pregnenolone has been described. The desmolase contains: NADPH dependent adrenodoxin reductase (AdxR), soluble adrenodoxin (Adx) and cytochrom P450 of the CYP 11 gene family. The crystal structure of the desmolase components, as well as the electron transportmechanism conducted by this complex, were recently established; the mechanisms which regulate the desmolase activity are fragmentally recognized, however, tissue specific regulatory systems exist. The main factor limiting desmolase activity, recently discovered, is the delivery of cholesterol from the outer to the inner mitochondrial membrane. This system depends on a permanent synthesis of cholesterol transporting protein StAR (in adrenals) or proteins with a similar function in other tissues (eg. placenta).
Summary The lysosomal membrane delimits the interial part of lysosomes containing the enzymes capable of degrading practically all cellular macromolecules, and actively participates as well in establishing an efficient environment for the degradation processes. 1. H+-ATPase - the main membrane protein complex ensures the low pH environment needed for efficient activity of lysosomal hydrolases. 2. Glycoproteins lamp2a are involved in specific degradation of proteins containing the KFERQ sequence (chaperone mediated autophagy) under condition of stress and starvation. 3. Specific lysosomal membrane proteins participate in export from and import to of low molecular weight compounds such as amino acids, dipeptides, sugars, nucleosides, or ions.