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Selection for high erucic acid content in winter oilseed rape (Brassica napus L.) on microspore-derived embryos

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Cegielska-Taras T., Szala L., Naleczynska A., Kolodziej K., Orodowczyk M.
Journal of Applied Genetics
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1999
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vol. 40
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issue 4
305-315
EN
Application of doubled-haploid (DH) techniques combined with a method of early selection for erucic acid in cotyledons of microspore-derived embryos (MDEs) from three hybrids of winter oilseed rape (high erucic acid and low glucosinolates) was described. Comparisons of erucic acid content between MDEs and seeds from the same homozygous line DH-Gr85 demonstrated that seed-specific fatty acid composition can be fully stimulated in MDEs after 18 days of culture on the NLN-medium with 0.8% agar and 2% sucrose with or without 10 mM ABA. The erucic acid content (percentage of the total fatty acid content) of cotyledons of embryos from three hybrids and of the seeds derived from plants regenerated from the remaining parts of the embryos were highly correlated (r = 0.78**).
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