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Application of pollen for studies on genetic diversity with the using of the Andean lupin (Lupinus mutabilis Sweet) as example

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Sawicka-Sienkiewicz E., Augustyniak H., Pietkiewicz G., Bartkowiak S.

Biotechnologia

2001

issue 1

101-111

The cultivated American species Lupinus mutabilis was selected in order to assess its possibilities of being introduced as an alternative high protein crop in Polish conditions. The realized programme involved identification of earlier maturing and higher yielding genotypes as well as interspecific hybridization with lupins belonging to section Albus, which includes the oldest cultivated species Lupinus albus. Recent reports (1) on determination of DNA polymorphism by the PCR method indicate usefulness of direct application of small amounts of pollen. The advantage of this method is the precisely determined and physiologically stable material used for the analysis. Furthermore, pollen can be preserved above the hygroscopic substance at ?20oC for a long time and small amounts of pollen (20-100 grains) can be used for the analysis without any damage to the plant material. For PCR reaction, ten selected primers were used. A polyacrylamide gel comparison of the PCR products revealed that random primers as well as primers which amplified a fragment of the whole gene can be applied to analyse the DNA of polymorphism of L. mutabilis and L. albus. The conclusion is that it is possible to differentiate within both groups of species by application of pollen to DNA analysis.

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1 Biotechnologia

1 2001

1 Augustyniak H.

1 Bartkowiak S.

1 Pietkiewicz G.

1 Sawicka-Sienkiewicz E.

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Application of pollen for studies on genetic diversity with the using of the Andean lupin (Lupinus mutabilis Sweet) as example