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Optical Fiber Sensors for Point Investigation οf Cancer Tissues

100%
Beres-Pawlik E., Gasiorek K., Kulas Z., Rzaca M., Czarnecki R.
Acta Physica Polonica A
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2009
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vol. 116
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issue 3
254-256
EN
The paper presents optical fiber sensors for point investigation of cancer tissues. The construction of several sensors has been described, including selection of proper optical fiber as well as the construction of measuring head. Being part of medical endoscopes, presented sensors can be applied to investigate autofluorescence spectra in various tissue areas. The level of autofluorescence intensity indicates the stage of disease advancement. The paper presents the results of investigating autofluorescence spectra for different cancer tissues. Various areas of cancer tissues have been investigated in order to determine the level of disease advancement. In the paper new constructions of fluorescent fiber sensors are presented. The sensors were used to investigate human and animal normal and tumor tissues. The fiber sensors, possible to use in videoendoscopy, are very small. The comparative analysis of fluorescent spectra of normal and tumor tissues is presented.
2
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Structural Characterization of Carane Derivative Stereoisomers - Potent Local Anesthetics

86%
Grochowski J., Serda P., Pasenkiewicz-Gierula M., Czarnecki R., Librowski T., Lochyński S., Frąckowiak B.
Acta Physica Polonica A
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2002
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vol. 101
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issue 5
665-674
EN
The paper reports on structural investigation and phase analysis of a newly synthesized potent local anesthetic with chiral molecular structure. Absolute structure and absolute configuration on four chiral centres was determined using microcrystalline single-crystal diffraction with anomalous scattering of X-ray radiation azimuthal scan technique. Phase analysis for new compound (KP23SS) and its epimer (KP23RS) was carried out using classical and synchrotron radiation powder diffraction. Enantiopurity of the bulk material was verified for both isomers by comparison of experimental and simulated high-resolution powder diffraction diagrams. The presence of two new polymorphic phases of KP23RS was documented. Comparative conformational analysis was carried out using differential Fourier synthesis and least-squares molecule overlap technique. A model of epimeric disorder was discussed for the homochiral phase.
3
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Point Investigation Method for Cancer Changed Tissues

86%
Kłonowski Ł., Kulas Z., Bereś-Pawlik E., Rząca M., Czarnecki R., Grzebieniak Z.
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EN
In this paper we describe the method of point investigation for cancer changed tissues with application of fluorescence phenomenon. The measurements have been made using a specially constructed scanning setup and fiber sensors. The experiment with investigation of endogenous fluorescence has been made on different types of slide tissues (e.g. breast and intestine tumor or precancerous and pathological skin tissues). The obtained spectral characteristics of fluorescence, with typical intensity peaks in 480-520 nm range, have explicitly outlined healthy and pathologically changed areas. The intensity of detected fluorescence determines the evaluation of disease advancement. Moreover, the ability to scan the surface of a tissue sample with constantly moving step of scanning setup in X-Y axis allows us to present the results in a spatial distribution of fluorescence intensity.
4

Association od the DdeI growth hormone gene polymorphism with some performance traits in Polish Large White and Czech Large White ? Polish Large White pigs

59%
Putnova l., Krenkova K. L., Krenkova L., Vrtkova V. I., Vrtkova I., Dvorak D. J., Dvorak J., Pietruszka P. A., Pietruszka A., Czarnecki C. R., Czarnecki R.
Journal of Applied Genetics
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2001
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vol. 42
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issue 3
317-324
EN
. The genotypes of growth hormone gene polymorphisms (GH-DdeI, GH-MspI, GH-HaeII, GH-ApaI, GH-CfoI) were determined in 78 pigs [Czech Large White sires (CLWsire line) ? Polish Large White (PLW) sows, Polish Large White sires ? Polish Large White sows], by the PCR-RFLP method. Preliminary studies found only GH DdeI polymorphism to be associated with performance traits. The associations of this polymorphism with growth and carcass traits were investigated. The linear model included the effects of candidate genes, genetic groups, sex and linear covariables of age at slaughter and body weight at weaning. The DdeI polymorphism of the GH gene showed associations with carcass length (P 0.05), average daily gain from birth to weaning (P 0.05) and average daily gain from weaning to slaughter (P 0.05). The association of GH genotypes with feed conversion was near significance. Sex influenced average daily gain from birth to weaning. The genetic groups (Czech or Polish sires) improved fat thickness at sacrum point 3, average daily gain from birth to weaning, lean meat content, weight of chop, weight of shoulder, weight of neck and average daily gain from weaning to slaughter. The regression on age at slaughter and on body weight at weaning influenced the majority of production traits.
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