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Rapid assessment of early embryo development in some wide crosses of cereals

100%

Slusarkiewicz-Jarzina A., Ponitka A., Wojciechowska B., Wozna J.

Journal of Applied Genetics

2000

vol. 41

issue 2

75-80

The cleared-ovule technique was evaluated for rapid examination of early embryo development in selfed (barley, wheat) and crossed (barley x rye and reciprocal barley x wheat) material. The pistils were fixed in FAA and the isolated ovules were cleared in methyl salicylate. The development of embryos and endosperm were observed at 24, 48, 72 and 96 hours after pollination. The embryo development rate in barley x rye crosses was mostly similar but in some cases delayed or slightly faster than in control mother cultivars. In contrast to barley x rye crosses, in the reciprocal barley x wheat crosses the frequency of embryos was lower. The study shows that the technique of cleared-ovule in methyl salicylate may be applied for rapid assessment of early embryo and endosperm development in cereal plants. In comparison with traditional sectioning methods the clear ovule technique is simpler, more efficient and quicker.

The influence of various in vitro culture conditions on androgenetic embryo induction and plant regeneration from hexaploid triticale (x Triticosecale Wittm.)

76%

Ponitka A., Slusarkiewicz-Jarzina A., Wedzony M., Marcinska I., Wozny J., Wozna J.

Journal of Applied Genetics

1999

vol. 40

issue 3

165-174

The effect of sucrose and maltose in culture media PII, C17 and MN6 on androgenetic embryo formation was investigated in seven F2 triticale hybrid progenies. In all genotypes, the highest number of androgenetic embryos (89.8-320.6/100 anthers) was formed on medium C17 containing maltose. Also the highest number of green plants (6.17/100 embryos) was regenerated from embryos obtained on PII with sucrose. The effect of the physical environment (temperature, light) in the first week of embryo culture on the regeneration medium was tested. The highest rate of green plants per 100 embryos (2.5-11.8) was obtained from incubation at 22oC in the dark. Key words: androgenetic embryos, incubation temperature, media, plant regeneration, triticale.

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2 Journal of Applied Genetics

1 2000

1 1999

2 Ponitka A.

2 Slusarkiewicz-Jarzina A.

2 Wozna J.

1 Marcinska I.

1 Wedzony M.

1 Wojciechowska B.

1 Wozny J.

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Rapid assessment of early embryo development in some wide crosses of cereals

The influence of various in vitro culture conditions on androgenetic embryo induction and plant regeneration from hexaploid triticale (x Triticosecale Wittm.)