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Rapid flow injection electrochemical detection of 3,3′,4,4′ tetrachlorobiphenyl using stabilized lipid membranes with incorporated sheep antibody

100%
Nikolelis D., Psaroudakis N., Michaloliakos A., Nikoleli G.-P., Scoullos M.
Open Chemistry
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2013
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vol. 11
|
issue 2
320-323
EN
An electrochemical biosensor based on a supported polymerized lipid film with incorporated sheep anti-3,3′,4,4′ tetrachlorobiphenyl (PCB congener 77) antibody using flow injection analysis was developed. The polymerized lipid film contained 85% (w/w) dipalmitoylphosphatidylcholine (DPPC) and 15% (w/w) dipalmitoylphosphatidic acid (DPPA), methacrylic acid, ethylene glycol dimethacrylate, AIBN and sheep anti-congener 77 antiserum. Congener 77 was injected into flowing carrier electrolyte and the flow stopped to detect the antigen. These membranes gave only a single transient proportional to log [congener 77] from 10−8 to 10−5 M, with a detection limit of ca. 10−8 M. A membrane containing 35% (w/w) DPPA was used to examine regeneration. The maximum number of cycles was about 5. [...]
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Potentiometric cholesterol biosensing application of graphene electrode with stabilized polymeric lipid membrane

73%
Nikoleli G.-P., Ibupoto Z., Nikolelis D., Likodimos V., Psaroudakis N., Tzamtzis N., Willander M., Hianik T.
Open Chemistry
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2013
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vol. 11
|
issue 9
1554-1561
EN
Abstract A novel potentiometric cholesterol biosensor has been fabricated through the immobilization of the stabilized polymeric lipid membrane onto graphene electrode. The stabilized polymeric lipid membrane is composed of cholesterol oxidase enzyme and polymerization mixture; which holds paramount influence on the properties of the cholesterol biosensor. The presented biosensor reveals an appreciable reproducibility, good selectivity and high sensing capability with a linear slope curve of ∼64 mV per decade. The strong biocompatibility among stabilized polymeric lipid membranes and human biofluids provides the possibility to use for real blood samples and other biological applications. Graphical abstract [...]
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