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1
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UV- and MMS-induced mutagenesis of lO(am)8 phage under nonpermissive conditions for phage DNA replication.

100%
Krwawicz J., Czajkowska A., Felczak M., Pietrzykowska I.
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2003
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vol. 50
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issue 4
921-939
EN
Mutagenesis in Escherichia coli, a subject of many years of study is considered to be related to DNA replication. DNA lesions nonrepaired by the error-free nucleotide excision repair (NER), base excision repair (BER) and recombination repair (RR), stop replication at the fork. Reinitiation needs translesion synthesis (TLS) by DNA polymerase V (UmuC), which in the presence of accessory proteins, UmuD', RecA and ssDNA-binding protein (SSB), has an ability to bypass the lesion with high mutagenicity. This enables reinitiation and extension of DNA replication by DNA polymerase III (Pol III). We studied UV- and MMS-induced mutagenesis of λO(am)8 phage in E. coli 594 sup+ host, unable to replicate the phage DNA, as a possible model for mutagenesis induced in nondividing cells (e.g. somatic cells). We show that in E. coli 594 sup+ cells UV- and MMS-induced mutagenesis of λO(am)8 phage may occur. This mutagenic process requires both the UmuD' and C proteins, albeit a high level of UmuD' and low level of UmuC seem to be necessary and sufficient. We compared UV-induced mutagenesis of λO(am)8 in nonpermissive (594 sup+) and permissive (C600 supE) conditions for phage DNA replication. It appeared that while the mutagenesis of λO(am)8 in 594 sup+ requires the UmuD' and C proteins, which can not be replaced by other SOS-inducible protein(s), in C600 supE their functions may be replaced by other inducible protein(s), possibly DNA polymerase IV (DinB). Mutations induced under nonpermissive conditions for phage DNA replication are resistant to mismatch repair (MMR), while among those induced under permissive conditions, only about 40% are resistant.
2
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Anthropometric and cardio-respiratory indices and aerobic capacity of male and female students

100%
Czajkowska A., Mazurek K., Lutosławska G., Żmijewski P.
Biomedical Human Kinetics
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2009
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vol. 1
47-51
EN
Study aim: To assess the relations between anthropometric and cardio-respiratory indices, and aerobic capacity of students, differing in the level of physical activity, under resting and exercise conditions.Material and methods: A group of 87 male and 75 female students volunteered to participate in the study. Their physical activity was evaluated by Seven-Day Physical Activity Recall questionnaire. Anthropometric (body height and mass, body fat content, BMI and WHR) and physiological indices (heart rate, blood pressure, O2max, CO2 and minute ventilation) were recorded. Body fat content was determined using a BIA device; respiratory data were recorded in a cycle ergometer test. The subjects were classified by their O2max values into ‘high’ or ‘low’ categories, the cut-off values of lower and upper quartiles serving as criteria.Results: Male and female students expended 10.2 ± 4.6 and 8.4 ± 5.3 kcal/kg/day, respectively, the O2max amounting to 48.4 ± 6.4 and 41.1 ± 4.7 ml/kg/min, respectively. Subjects having high O2max had significantly higher energy expenditure on physical activities, fat-free mass, body water content and maximal ventilation, and lower body mass, BMI, body fat content, resting heart rate and diastolic pressure.Conclusions: When investigating into the relationships between physical activity and physiological features, the latter ought to be related to O2max rather than to energy expenditure which may depend on other than physiological variables.
3
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The index of insulin resistance (FIRI) is not associated with plasma homocysteine levels in young, non-obese healthy men and women

88%
Czajkowska A., Lutosławska G., Mazurek K., Ambroszkiewicz J., Żmijewski P.
Biomedical Human Kinetics
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2011
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vol. 3
14-17
EN
Study aim: To evaluate plasma homocysteine (Hcy), insulin and glucose levels in blood and the insulin resistance index (FIRI) in young, healthy non-obese men and women.Material and methods: A total of 152 young, healthy, non-obese (BMI<30) men (n = 81) and women (n = 71) participated in the study. The following substances were assayed in blood using commercial kits: total plasma homocysteine by fluorescence polarisation immunoassay, plasma glucose - by the oxidase method, and insulin by radioimmunoassay using monoclonal antibodies. From the latter two, the index of insulin resistance (FIRI) was computed.Results: Mean plasma homocysteine concentration in men was significantly (p<0.001) higher than in women (10.3 ± 3.0 and 8.4 ± 2.4 μmol/l, respectively) and that of FIRI was significantly (p<0.001) lower than in women (1.310 ± 0.483 and 1.437 ± 0.420, respectively). Neither in men nor in women were plasma homocysteine concentrations correlated with FIRI.Conclusions: Although no association between circulating homocysteine and FIRI was found in young, non-obese men and women, the existence of such association in Type 2 diabetes cannot be ruled out.
4
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Cardio-pulmonary fitness and anthropometric features in men aged 50+ trained in long-distance running and those not trained

88%
Witek K., Mazurek K., Mróz A., Czajkowska A., Kęska A.
Biomedical Human Kinetics
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2014
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vol. 6
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issue 1
EN
Study aim: the aim of study was assessment somatic features and selected cardio-ventilatory indices in men above 50 years old with different physical activity levels. Materials and methods: the study included 55 men on average aged 54.5 ± 4.32, classified to the trained group (T), n = 33, and not trained group (NT), n = 22. Total physical activity was assessed using the SDPAR Questionnaire. Measurements: anthropometric indices, ventilatory indices and VO2 peak, hemodynamic indices and fatigue using Borg scale during exercise maximal test. Results: daily energy expenditures for sport and recreation differ significantly between T and NT groups, 6.82 METs vs. 0.2 METs, respectively (p < 0.001). Men in T and NT groups significantly differ in somatic features, and cardio-pulmonary indices including: time of physical tests effort (s) 1103 ± 193 vs. 681 ± 328; max speed (km/h) 14 ± 1.5 vs. 10 ± 1.4, VE peak 135.9 ± 21.17 l/min vs. 112.9 ± 21.49 l/min; VO2 peak 61.8 ± 8.83 ml/kg/min vs. 41.9 ± 8.55 ml/kg/min; HR rest (beat/min) 69 ± 16.95 vs. 83 ± 14.48; HR peak (beat/min) 171 ± 1.23 vs. 163 ± 15.28; SV peak (ml) 162 ± 24.23 vs. 135 ± 33.22; CO peak 27.4 ± 4,3 vs. 21.6 ± 5.17. Conclusions: men aged 50+ who practice running training differ favourably and significantly from men not trained in anthropometric as well as cardio-ventilatory indices. Significant correlations were found between DEE and FAT(%), VO2 peak (ml/kg/min), SV peak (ml) and COpeak (l/min) as well between CO peak and VO2peak was record linear relationship (r = 0.56).
5
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Physical fitness women over 60 years of age participating in the activities of the University of the Third Age

76%
Zdrodowska A., Wiszomirska I., Niemierzycka A., Czajkowska A., Magiera A., Słoń M.
Advances in Rehabilitation
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2012
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vol. 26
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issue 3
19-25
EN
Introduction: Morphological and functional changes in cells, tissues and organs causing characteristic for old age reduction of the efficiency of all organs, and therefore decrease in the level of physical fitness. To determine its level, to find areas of weakness and select optimal physical activity programs that will improve the quality of life and health of older people it is necessary to multi-dimensional assess the level of physical fitness. The aim of this study is to evaluate the fitness level of students of the University of the Third Age in Warsaw Almamer with Fullerton Functional Fitness Test in relation to American standards. Material and methods: The study was conducted on a 40-person group of students of the University of Third Age Almamer in Warsaw, at the age of 60-70 years. Mobility assessment was based on a comparison of results obtained by the Fullerton Functional Fitness Test with standards developed by the authors test for the U.S. population. Results: In all trials conducted with Fullerton Test women got positive results after normalization to the mean and standard deviation of the U.S. population peers. The greatest variation among the test subjects compared to American standards test result was observed in flexion of the forearm. Conclusions: The tested listeners of UTW characterized a good level of physical fitness Fullerton The test evaluated in relation to American standards. According to the authors, developing standards for the test used in Polish conditions would contribute to a more reliable assessment of people over 60 years old.
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