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Cloning and partial characterization of a gene in Bombyx mori homologous to a human adiponectin receptor

100%

Zhu M., Chen K., Wang Y., Guo Z., Yin H., Yao Q., Chen H.

2008

vol. 55

issue 2

241-249

In this study, we report the cloning and characteristics of an adiponectin-like receptor gene from Bombyx mori (BmAdipoR) with highly conserved deduced amino-acid sequences and similar structure to the human adiponectin receptor (AdipoR). Structural analysis of the translated cDNA suggested it encoded a membrane protein with seven transmembrane domains. BmAdipoR was found to be expressed in multiple tissues and highly expressed in Malpighian tubules, fat body and testis. BmNPV (Bombyx mori nucleopolyhedrovirus) bacmid system combined with confocal microscopy revealed that BmAdipoR was targeted to the cell membrane. We also found that infection with BmNPV did not have an effect on BmAdipoR mRNA quantity in the midgut of susceptible Bombyx mori strain (306) at 48 h, but BmAdipoR mRNA quantity increased significantly at 72 h. We concluded that BmAdipoR gene was a membrane protein ubiquitously expressed in Bombyx mori tissues and that its expression was altered by treating with BmNPV.

Molecular cloning, expression and characterization of Bmserpin-2 gene from Bombyx mori

84%

Pan Y., Xia H., Lü P., Chen K., Yao Q., Chen H., Gao L., He Y., Wang L.

issue 4

671-677

Serpins are a broadly distributed family of protease inhibitors. In this study, the gene encoding Bombyx mori serpin-2 (Bmserpin-2) was cloned and expressed in E. coli. The Bmserpin-2 cDNA contains a 1125 bp open reading frame (ORF). The deduced protein has 374 amino-acid residues, contains a conserved SERPIN domain and shares extensive homology with other invertebrate serpins. RT-PCR analysis showed that Bmserpin-2 was expressed in all developmental stages of B. mori larvae and various larval tissues. Subcellular localization analysis indicated that Bmserpin-2 protein was located in the cytoplasm. Interestingly, real-time quantitative PCR revealed that the expression of Bmserpin-2 in the midgut of susceptible B. mori strain 306 significantly increased at 72 hours post inoculation (hpi) when infected with BmNPV. However, there was no significant increase of the Bmserpin-2 expression in resistant strain NB infected with BmNPV. Thus, our data indicates that Bmserpin-2 may be involved in B. mori antiviral response.

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2 Acta Biochimica Polonica

1 2009

1 2008

2 Chen H.

2 Chen K.

2 Yao Q.

1 Gao L.

1 Guo Z.

1 He Y.

1 Lü P.

1 Pan Y.

1 Wang L.

1 Wang Y.

1 Xia H.

1 Yin H.

1 Zhu M.

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Cloning and partial characterization of a gene in Bombyx mori homologous to a human adiponectin receptor

Molecular cloning, expression and characterization of Bmserpin-2 gene from Bombyx mori