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EN
Microcuttings of chrysanthemum radiomutants were irradiated in vitro with gamma rays with a dose of 15 Gy. The adventitious shoots' regeneration on MS medium was applied. The medium used was supplemented with plant growth regulators: 2 mg l-1 IAA and 0.6 mg l-1 BAP. An effect of radiation on the numbers of adventitious shoots regenerated from irradiated leaf explants was determined. 'Lady Vitroflora' produced substantially more adventitious shoots per one explant than 'Lady Apricot'. The ionizing radiation clearly reduced leaf explants regeneration capacity. The rate of adventitious shoots regeneration depended on the cultivar and was always higher than that for non-irradiated explants.
EN
Success of breeding programmes using adventitious shoots technique often depends on the regeneration efficiency. The aim of this study was to investigate the influence of topophysical position of explants on the efficiency of adventitious shoots regeneratio . Uniform, single shoots of chrysanthemum 'Satinbleu' propagated in vitro were divided equally into three topophysical zones: distal, central and proximal. Two leaves and two internodes were isolated from each zone. The explants were cultured for 12 weeks on MS medium supplemented with 0.6 mg l-1 BAP and 2.0 mg l-1 IAA. Due to the highest adventitious shoots regeneration capability and the earliest formation of the shoots, the most suitable explants for breeding programmes for chrysanthemum are internodes excised from distal part of in vitro plantlet.
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