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1

The role of mutagenesis in the modification of the fatty acid profile of oilseed crops

100%
Velasco L., Perez-Vich B., Fernandez-Martinez J. M.
Journal of Applied Genetics
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1999
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vol. 40
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issue 3
185-209
EN
Mutagenesis has played a major role in the development of novel variation for the fatty acid profile of oilseed crops. Although the first successful experiments were started in the 70s, most of the achievements in this field have been reported during the 80s and 90s. This paper summarizes the role of mutagenesis in the modification of the fatty acid profile of oilseed crops, the genetic basis of such modifications, and the consequences for plant breeding.
2

Transferability, amplification quality, and genome specificity of microsatellites in Brassica carinata and related species

100%
Marquez-Lema A., Velasco L., Perez-Vich B.
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vol. 51
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issue 2
123-131
EN
No information is available on the transferability and amplification quality of microsatellite (SSR) markers of the public domain in Brassica carinata A. Braun. The objective of the presented research was to study the amplification of a set of 73 SSRs from B. nigra (L.) Koch and B. napus L. in B. carinata, and to compare the results with those obtained in the amplification of the same markers in other Brassica species of the U triangle. This set of SSRs from B. nigra (B genome) and B. napus (AC genome) allows the identification of the 3 basic genomes of the Brassica species tested. 94.3% of the SSR markers from B. nigra and 97.4% of those from B. napus amplified SSR-specific products in B. carinata. Very high-quality amplification with a strong signal and easy scoring in B. carinata was recorded for 52.8% of the specific loci from B. nigra SSRs and 59.3% of the specific loci from B. napus SSRs, compared to 66.7% in B. nigra and 62.8% in B. napus. Genome specificity and amplification quality of B. nigra and B. napus SSR markers in the 6 species under study is reported. High-quality transferable SSR markers provide an efficient and cost-effective platform to advance in molecular research in B. carinata.
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