Results found: 3

Number of results on page

Search results

Sort By:

Limit search:

Amyloid beta protein affects poly(ADP-ribose) polymerase activity in PC-12 cells in culture

100%

Strosznajder R. P., Banasik M.

Acta Neurobiologiae Experimentalis

2000

vol. 60

issue 2

215

The effects of organic solvents on poly(ADP-ribose) polymerase-1 activity: implications for neurotoxicity

61%

Banasik M., Stedeford T., Strosznajder R. P., Persad A. S., Tanaka S., Ueda K.

Acta Neurobiologiae Experimentalis

2004

vol. 64

issue 4

467-473

Poly(ADP-ribose) polymerase-1 (PARP-1; EC 2.4.2.30), also termed as poly(ADP-ribose) synthetase, is a key enzyme in the recognition and repair of damaged DNA. Several conditions (e.g., ischemia-reperfusion or chemical-induced injury) have been shown to overactivate PARP-1, causing neurodegeneration and necrotic or apoptotic cell death from NAD+ and ATP depletion. In contrast, inhibitors of PARP-1 have been shown to have a neuroprotective effect by ameliorating this response. The purpose of this study was to determine the effects of three routinely used organic solvents (ethanol, methanol, and dimethyl sulfoxide (DMSO)) on the activity of purified PARP-1. A dose-response was examined with each of these solvents. A 112% and 82% increase in PARP-1 activity was observed with 15% ethanol and 20% methanol, respectively. In contrast, a near 20% decrease in the activity was observed with 4% DMSO. Kinetic analysis revealed that the maximal velocity remained unchanged with increasing concentrations of DMSO up to 20%, indicating that DMSO is a competitive inhibitor of PARP-1. Thus, PARP-1 inhibition by DMSO depends on NAD+ concentration and in some pathological processes might be significant even at low DMSO concentrations. Our findings suggest that the interpretation of data from dose-response studies obtained when using common organic solvents may be dramatically skewed, either exaggerating the inherent toxicity of the compound or masking its potential for damage.

Frequencies of killer immunoglobulin-like receptor genotypes influence susceptibility to spontaneous abortion

42%

Nowak I., Malinowski A., Tchorzewski H., Barcz E., Wilczynski J. R., Grybos M., Kurpisz M., Luszczek W., Banasik M., Reszczynska-Slezak D., Majorczyk E., Wisniewski A., Senitzer D., Sun D. Y., Kusnierczyk P.

Journal of Applied Genetics

2009

vol. 50

issue 4

391-398

Natural killer (NK) cells are the most abundant lymphocyte population in the decidua. These cells express killer immunoglobulin-like receptors (KIRs), which upon recognition of HLA class I molecules on trophoblasts may either stimulate NK cells (activating KIRs) or inhibit them (inhibitory KIRs) to produce soluble factors necessary for the maintenance of pregnancy. KIR genes exhibit extensive haplotype polymorphism; individuals differ in both the number and kind (activating vs. inhibitory) of KIR genes. This polymorphism affects NK cell reactivity and susceptibility to diseases, including gynecological disorders. Therefore we KIR-genotyped 149 spontaneously aborting women and 117 control multiparae (at least 2 healthy-born children). Several genotypes (i.e. combinations of various KIR genes) were differently distributed among the patients and control subjects. Differences were observed in the numbers and the ratios of activating to inhibitory KIRs between patients and healthy women: (i) genotypes containing 6 activating KIR genes were less frequent and those containing 6 inhibitory KIR genes were more frequent in patients than in control subjects, and (ii) an excess of inhibitory KIRs (activating-to-inhibitory KIR gene ratios of 0.33 to 0.83) was associated with miscarriage, whereas ratios close to equilibrium (0.86?1.25) seemed to be protective. In addition, the results suggest for the first time that sporadic and recurrent spontaneous abortions as well as miscarriage in the presence or absence of autoantibodies may have different KIR genotypic backgrounds.

Refine search results

2 Acta Neurobiologiae Experimentalis

1 Journal of Applied Genetics

1 2009

1 2004

1 2000

3 Banasik M.

2 Strosznajder R. P.

1 Barcz E.

1 Grybos M.

1 Kurpisz M.

1 Kusnierczyk P.

1 Luszczek W.

1 Majorczyk E.

1 Malinowski A.

1 Nowak I.

1 Persad A. S.

1 Reszczynska-Slezak D.

1 Senitzer D.

1 Stedeford T.

1 Sun D. Y.

1 Tanaka S.

1 Tchorzewski H.

1 Ueda K.

1 Wilczynski J. R.

1 Wisniewski A.

Search results

Amyloid beta protein affects poly(ADP-ribose) polymerase activity in PC-12 cells in culture

The effects of organic solvents on poly(ADP-ribose) polymerase-1 activity: implications for neurotoxicity

Frequencies of killer immunoglobulin-like receptor genotypes influence susceptibility to spontaneous abortion