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Human hAtg2A protein expressed in yeast is recruited to preautophagosomal structure but does not complement autophagy defects of atg2Δ strain

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Romanyuk D., Polak A., Maleszewska A., Sieńko M., Grynberg M., Żołądek T.

2011

vol. 58

issue 3

365-374

Yeast ScAtg2, an autophagy-related protein, is highly conserved in other fungi and has two homologues in humans, one of which is hAtg2A encoded by the hATG2A/KIAA0404 gene. Region of homology between Atg2 and hAtg2A proteins comprises the C-terminal domain. We used yeast atg2D strain to express the GFP-KIAA0404 gene, its fragment or fusions with yeast ATG2, and study their effects on autophagy. The GFP-hAtg2A protein localized to punctate structures, some of which colocalized with Ape1-RFP-marked preautophagosomal structure (PAS), but it did not restore autophagy in atg2Δ cells. N-terminal fragment of Atg2 and N-terminal fragment of hAtg2A were sufficient for PAS recruitment but were not sufficient to function in autophagy. Neither a fusion of the N-terminal fragment of hAtg2A with C-terminal domain of Atg2 nor a reciprocal fusion were functional in autophagy. hAtg2A, in contrast to yeast Atg2, did not show interaction with the yeast autophagy protein Atg9 but both Atg2 proteins showed interaction with Atg18, a phospholipid-binding protein, in two-hybrid system. Moreover, deletion of ATG18 abrogated PAS recruitment of hAtg2A. Our results show that human hAtg2A can not function in autophagy in yeast, however, it is recruited to the PAS, possibly due to the interaction with Atg18.

The correlation between selected anthropometric indices and BIA - based body fat measures in nursing home women aged 80+ years

84%

Zarzeczny R., Nawrat - Szołtysik A., Polak A., Maliszewski J., Kiełtyka A., Matyja B., Dudek M., Zborowska J., Wajdman A.

The aims of the study were to assess whether commonly used anthropometric indices are associated with body fat measures obtained by Bioel ectrical Impedance Analysis (BIA) method, and to determine the best anthropometric predictor of BIA - based body fat percentage (%Fat) and visceral fat rating (VFR) in elderly women. The sample consisted of 24 women aged 80 years and older, the residents of long - term care facilities in Upper Silesia (Poland). All women were subjected to standard anthropometric measurements including the following variables: body weight, body height, waist circumference, hip circumference and neck circumference. On the basis o f these measures Body Mass Index (BMI) as well as waist - to - hip ratio (WHR) were calculated. The subjects’ %Fat and VFR were determined by body composition analyzer TANITA BC 420MA (Japan). Pearson’s correlation coefficients were used to quantify the relati onships between variables. Stepwise multiple regression analysis with backward elimination was performed to identify possible predictors. The basic characteristics of the investigated subjects were as follows (mean±SD): age - 85.5±3.7 years; body weight - 60.4±11.6 kg; body height - 150.6±7.6 cm; BMI - 26.6±4.6 kg/m 2 ; %Fat - 31.3±9.6%; VFR - 10.7±2.5. Both of BIA - based measures significantly, positively correlated with body weight, BMI and circumferences of waist, hip and neck (r values from 0.477 to 0.835) . The multiple regression analysis for %Fat revealed that the body weight was the only variable statistically significant (r 2 =0.414; p<0.001; SEE =7.503%), and for VFR the significant β coefficients were obtained for BMI and neck circumference ( 0.625±0.133 and 0.341±0.133, respectively) ( r 2 =0.754; p<0.001; SEE =1.313). Among popular anthropometric indices of body composition in the oldest old group o f women, body weight seems to be the best predictor of body fat percentage, and VFR could be predicted by BMI along with a neck circumference.

Pressure injury treatment with anodal and cathodal electrical stimulation in persons with nervous system injuries. A prospective, randomized, clinical study. Preliminary report.

68%

Polak A., Nawrat-Szołtysik A., Ickowicz T., Kucio E., Kasprzak K., Chlebek A., Etfer B., Pniowska M., Rajca J., Łagodzic S., Kania W.

vol. 21(3)

23-34

Wstęp: Elektrostymulacja (ES) jest zabiegiem rekomendowanym w leczeniu odleżyn ale metodyka zabiegów zapewniających najlepsze skutki lecznicze wymaga jeszcze ustalenia. Cel: Porównanie skuteczności ES katodowej i anodowej w leczeniu odleżyn II-IV stopnia. Projekt badawczy: Badanie eksperymentalne z randomizacją. Materiał i metody: 38 osób z odleżynami leczonych w Centrum Rehabilitacji na terenie Górnego Śląska zostało losowo podzielonych do grupy elektrostymulacji anodowej (AG/12 osób; średni wiek 52,83 lata), elektrostymulacji katodowej (KG/13 osób; średni wiek 52,00 lata) i elektrostymulacji placebo (PG/13 osób; średni wiek 54,46 lata). U wszystkich chorych stosowano standardowe leczenie odleżyn. Dodatkowo w AG i KG zastosowano elektrostymulację wysokonapięciową prądem pulsującym (high-voltage monophasic pulsed current − HVMPC) (impulsy podwójne szpiczaste, 154 μs; 100 Hz; 0,36 A; 360 μC/s) przez 50 minut dziennie, pięć dni w tygodniu, przez 8 tygodni. W AG elektrodą leczniczą była anoda, a w KG – katoda. W PG wykonywano symulowaną elektrostymulację. Obie elektrody były układane na wilgotnej gazie. Elektrodę leczniczą układano na powierzchni rany a zamykającą obwód na zdrowej skórze w odległości przynajmniej 15 cm od rany. Pole powierzchni odleżyn było mierzone przed leczeniem oraz po zakończeniu każdego kolejnego tygodnia terapii. Wyniki: W grupach AG i KG powierzchnia odleżyn zmniejszyła się odpowiednio o 73,68% (SD 28,03) i 76,02% (SD 17,51). Wyniki te były znamiennie statystycznie większe niż w PG (44,20%; SD 20,86). Wyniki uzyskane w AG i KG nie różniły się znamiennie statystycznie. Wnioski: ES wysokonapięciowa anodowa i katodowa w podobnym stopniu przyczyniają się do zmniejszenia odleżyn II-IV stopnia.

Introduction: Electrical stimulation (ES) is a treatment recommended for pressure injuries (PIs), but an optimal protocol methodology for wound treatment has not yet been established. Objective: Comparing the effectiveness of cathodal and anodal ES in the treatment of category II-IV pressure injuries. Research project: Experimental trial with randomization. Material and methods: 38 individuals with pressure wounds treated at the Rehabilitation Centre in the region of Upper Silesia were randomly divided into the anodal ES group (AG/12 people, mean age 52.83 years), cathodal ES group (CG/13 people, average age 52.00 years) and the ES placebo group (PG/13 people, average age 54.46 years). Standard pressure injury treatment was implemented in all patients. Additionally, in the AG and CG, ES with high-voltage monophasic pulsed current (HVMPC; twin-peak impulses; 154 μs; 100 pps; 0.36 A; 360 μC/s) was applied for 50 minutes a day, fi ve days a week, for 8 weeks. In the AG group, the healing electrode was an anode, while in the CG, cathodes were used. In the PG, sham ES was performed. Both electrodes were placed on moist gauze. The electrode for treatment was placed on the surface of the wound and the return electrode was positioned on healthy skin at least 15 cm from the PI edge. The surface area of the PIs was measured before and after each subsequent week of therapy. Results: In the AG and CG, the surface of the pressure injuries decreased by 73.68% (SD 28.03) and 76.02% (SD 17.51), respectively. These results were statistically signifi cantly higher than in the PG (44.20%, SD 20.86). The results obtained in AG and CG did not signifi cantly differ statistically. Conclusions: High-voltage anodal and cathodal ES cause a decrease in category II-IV pressure injuries to a similar extent.

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1 Acta Biochimica Polonica

1 Medical Rehabilitation

1 Physical Activity Review

1 2018

1 2016

1 2011

3 Polak A.

1 Chlebek A.

1 Dudek M.

1 Etfer B.

1 Grynberg M.

1 Ickowicz T.

1 Kania W.

1 Kasprzak K.

1 Kiełtyka A.

1 Kucio E.

1 Maleszewska A.

1 Maliszewski J.

1 Matyja B.

1 Nawrat - Szołtysik A.

1 Nawrat-Szołtysik A.

1 Pniowska M.

1 Rajca J.

1 Romanyuk D.

1 Sieńko M.

1 Wajdman A.

1 Zarzeczny R.

1 Zborowska J.

1 Łagodzic S.

1 Żołądek T.

Search results

Human hAtg2A protein expressed in yeast is recruited to preautophagosomal structure but does not complement autophagy defects of atg2Δ strain

The correlation between selected anthropometric indices and BIA - based body fat measures in nursing home women aged 80+ years

Pressure injury treatment with anodal and cathodal electrical stimulation in persons with nervous system injuries. A prospective, randomized, clinical study. Preliminary report.