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Upregulation of GRP78 and caspase-12 in diastolic failing heart

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Sun Y., Liu G., Song T., Liu F., Kang W., Zhang Y., Ge Z.

2008

vol. 55

issue 3

511-516

Background: The endoplasmic reticulum (ER) fulfills multiple cellular functions. Various stimuli can potentially cause ER stress (ERS). ERS is one of the intrinsic apoptosis pathways and apoptosis plays a critical role in hypertension. Glucose regulated protein 78 (GRP78) has been widely used as a marker for ERS and caspase-12 mediated apoptosis was a specific apoptotic pathway of ER. The expression of GRP78 and caspase-12 remains poorly understood in the diastolic heart failure resulting from hypertension. Methods: We used spontaneously hypertensive rats (SHRs) to establish a model of diastolic heart failure, and performed immunohistochemistry, western blot, and real-time PCR to analyze GRP78 and caspase-12. Results: We found that GRP78 and caspase-12 had enhanced expression at protein and mRNA levels. Conclusions: These results suggest that GRP78 and caspase-12 were upregulated in cardiomyocytes and ERS can contribute to cardiac myocyte apoptosis in the diastolic heart failure resulting from hypertension.

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1 Acta Biochimica Polonica

1 2008

1 Ge Z.

1 Kang W.

1 Liu F.

1 Liu G.

1 Song T.

1 Sun Y.

1 Zhang Y.

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Upregulation of GRP78 and caspase-12 in diastolic failing heart