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1

Mapping of sequence-specific markers and loci controlling preharvest sprouting and alpha-amylase activity in rye (Secale cereale L.) on the genetic map of an F2 (S120xS76) population

100%
Myskow B., Stojalowski S., Milczarski P., Masojc P.
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vol. 51
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issue 3
283-287
EN
Location of the loci that control preharvest sprouting and alpha-amylase activity in rye was studied based on intercross S120?S76, consisting of 110 genotypes of F2 and F3 progenies. The genetic map currently consists of 141 loci distributed in 11 linkage groups, covering a distance of 506.4 cM, and was enriched during this study with 24 sequence-specific markers (7 SCARs, 7 SSRs, and 10 STSs). The extended map was applied for composite interval mapping of the loci controlling preharvest sprouting and ?-amylase activity, revealing 3 significant QTLs for preharvest sprouting, located on chromosomes 3R, 5R and 6R (in 1999), and one QTL for ?-amylase activity found on chromosome 2R (in 2000).
2

Genetic regulation of alpha-amylase synthesis in rye (Secale cereale L.) grain

100%
Masojc P., Stojalowski S., Lapinski M., Miazga D.
Journal of Applied Genetics
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1996
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vol. 37
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issue 2
141-152
EN
Genetic analysis of two rye interline crosses and a set of wheat/rye chromosomal additoin lines was performed to reveal the mechanism underlying wide variation range of alpha-amylase activity in sound grain.THe long arm of chromosome 6R was found to be responsible for increased enzyme synthesiis during late stages of triticale grain maturation.Only nuclear genes seemed to control alpha-amylase activity, as reciprocal crosses between rye lines showed no maternal effects.Low enzyme activity showed complete dominance over high level of its synthesis.Segregation ratios, observed in F2 and BC1 crosses, indicated that recessive alleles at two independent duplicative loci underline intensive alpha-amylase production.
3

Rye SCAR markers for male fertility restoration in the P cytoplasm are also applicable to marker-assisted selection in the C cytoplasm

100%
Stojalowski S., Jaciubek M., Masojc P.
Journal of Applied Genetics
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2005
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vol. 46
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issue 4
371-373
EN
The study aimed at testing the usefulness of recently developed SCAR markers on rye (Secale cereale L.) chromosome 4R in hybrid breeding based on the C source of male sterility-inducing cytoplasm. Of 10 markers studied, 4 revealed polymorphisms between 2 inbred lines (544cms-C and Ot0-20) crossed to develop F2 and BC1 mapping populations. Analyses performed on 94 F2 and 93 BC1 plants allowed to extend a formerly constructed genetic map of chromosome arm 4RL. Three SCAR markers (SCP14M55, SCP15M55 and SCP16M58) were mapped in the vicinity of gene Rfc1, which restores male fertility in the C cytoplasm. The 3 tested SCAR markers proved to be effective in marker-assisted selection (MAS) for male fertility/sterility.
4

Determining the plasmotypic structure of rye populations by SCAR markers

88%
Stojalowski S., Kociuba M., Stochmal B., Kondziola M., Jaciubek M.
Journal of Applied Genetics
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2008
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vol. 49
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issue 3
229-232
EN
In rye (Secale cereale L.), 2 types of cytoplasmic male sterility are known: Pampa type (CMS-P) and Vavilovii type (CMS-V). As an alternative method to the conventional plasmotype-genotype interaction test, for identification of the cytoplasm type, the use of sequence-characterised amplified region (SCAR) markers was validated in this study. In over 2600 individual rye plants, representing 26 populations originating from Poland (18 cultivars), Iran (5 populations of primitive rye), and South America (3 populations), the cytoplasm type was determined by using a set of 3 SCAR markers. For about 10% of these individuals, the plasmotype-genotype interaction test was performed in parallel. The results of both tests were fully consistent. In the majority of the Polish populations, CMS-V was present, and only 4 populations contained CMS-P. Primitive Iranian populations contained predominantly normal cytoplasm, and only occasionally CMS-P was identified in them. South American populations displayed a mixture of normal cytoplasm, CMS-P and CMS-V. This work validates the use of SCAR markers as a reliable and quick method to determine the plasmotypic diversity of rye populations on a large scale.
5

New genetic map of rye composed of PCR-based molecular markers and its alignment with the reference map of the DS2 ? RXL10 intercross

76%
Milczarski P., Banek-Tabor A., Lebiecka K., Stojalowski S., Myskow B., Masojc P.
Journal of Applied Genetics
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2007
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vol. 48
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issue 1
11-24
EN
A new genetic map of rye, developed by using the 541 ? Ot1-3 F2 intercross, consists of 148 marker loci, including 99 RAPDs, 18 SSRs, 14 STSs, 9 SCARs and 7 ISSRs, and spans the distance of 1401.4 cM. To the 7 rye chromosomes, 8 linkage groups were assigned and compared with the reference map of the DS2 ? RXL10 F2 intercross by using 24 common markers. The 2 combined maps contain altogether 611 marker loci (70?109 per chromosome) and constitute a substantial source of information useful for further genomic studies in rye. From 21 to 37 RAPD marker loci are distributed randomly along each chromosome length and their total number for all 7 rye chromosomes is 177. This abundance of RAPD marker loci in the rye genetic map can be exploited for development of SCARs in regions containing important genes or QTL.
6

New genetic map of rye composed of PCR-based molecular markers and its alignment with the reference map of the DS2 ? RXL10 intercross

76%
Milczarski P., Banek-Tabor A., Lebiecka K., Stojalowski S., Myskow B., Masojc P.
Journal of Applied Genetics
|
2007
|
vol. 48
|
issue 3
11-24
EN
A new genetic map of rye, developed by using the 541 ? Ot1-3 F2 intercross, consists of 148 marker loci, including 99 RAPDs, 18 SSRs, 14 STSs, 9 SCARs and 7 ISSRs, and spans the distance of 1401.4 cM. To the 7 rye chromosomes, 8 linkage groups were assigned and compared with the reference map of the DS2 ? RXL10 F2 intercross by using 24 common markers. The 2 combined maps contain altogether 611 marker loci (70?109 per chromosome) and constitute a substantial source of information useful for further genomic studies in rye. From 21 to 37 RAPD marker loci are distributed randomly along each chromosome length and their total number for all 7 rye chromosomes is 177. This abundance of RAPD marker loci in the rye genetic map can be exploited for development of SCARs in regions containing important genes or QTL.
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