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1

Transgenic strawberry carrying maize IAA-glucose synthetase gene (iaglu)

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Wawrzynczak D., Sowik I., Michalczuk L.
Biotechnologia
|
2000
|
issue 4
103-105
EN
Strawberry leaves (Fragaria x ananassa Duch.) cv. Kaster were transformed with the aid of Agrobacterium tumefaciens, strain 4404, carrying pBIN19 plasmid with nptII gene and iaglu gene under the control of constitutive CaMV-35S promotor. The incorporation of the transgene was confirmed in PCR with iaglu-specific primers and its expression by Northern blot. The level of esterified IAA in the leaves of transgenic plants was higher and free IAA lower than in the leaves of control plants. Of 15 transgenic clones analysed, 10 have smaller leaves and 13 shorter petioles than control plants. It was also found that 8 transgenic strawberry clones started flowering 1 ? 2 weeks earlier than control plants and 5 clones did not form any runners.
2

The effect of transformation with iaglu gene on growth and development of trangenic strawberry in in vitro cultures

100%
Wawrzynczak D., Sowik I., Michalczuk L.
Biotechnologia
|
2004
|
issue 2
46-53
EN
The aim of the presented work was to study the effects of changes of endogenous indole-3-acetic acid (IAA) metabolizm on in vitro shoot proliferation and rhizogenesis of transgenic strawberry shoots carrying maize IAA-glucose synthase gene (iaglu). Four iaglu-transformed strawberry clones and nontransformed 'Kaster' shoots served as a plant material for the study. The analysis of free and conjugated IAA level in leaves of transgenic and control strawberry plants showed that iaglu-containing strawberry clones had significantly higher level of ester conjugated IAA, but the level of free hormone was only slightly decreased or comparable to the control plants. iaglu-transformed clones had significantly higher proliferation rate and formed more roots than the control shoots. One of the iaglu-transformed clones had significantly shorter and other two ? longer roots than the control plantlets.
3

In vitro shoot regeneration of strawberry as the source of somaclonal variants resistant to verticillium wilt?

100%
Sowik I., Wawrzynczak D., Michalczuk L.
|
EN
Somaclonal variants of strawberry resistant to verticillium wilt were selected by cocultivation of shoots of various cultivars obtained by both micropropagation and regeneration from a callus with a homogenate of 3-week-old liquid culture of mixed isolates of Veriticillium dahliae. There was a large variation in the resistance to the pathogen within a population of both micropropagated and regenerated from the callus shoots. After 70 days of cocultivation most of the shoots of susceptible cultivars were heavily damaged or dead, but there were some individuals that survived in a relatively good health. Those variants were transplanted on a new Boxus?s medium supplemented with 2 mg/l of benomyl to eliminate pathogen and their resistance to verticillium wilt is now tested.
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