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Comparative proteomic analysis of Bombyx mori hemolymph and fat body after calorie restriction

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Chen H., Li Y., Chen K., Yao Q., Li G., Wang L.

2010

vol. 57

issue 4

505-511

Calorie restriction (CR) is known to extend life span from yeast to mammals. To gain an insight into the effects of CR on growth and development of the silkworm Bombyx mori at protein level, we employed comparative proteomic approach to investigate proteomic differences of hemolymph and fat body of the silkworm larvae subjected to CR. Thirty-nine differentially expressed proteins were identified by MALDI TOF/TOF MS. Among them, 19 were from the hemolymph and 20 from the fat body. The hemolymph of the CR group contained two down-regulated and 17 up-regulated proteins, whereas the fat body contained 15 down-regulated and five up-regulated ones. These proteins belonged to those functioning in immune system, in signal transduction and apoptosis, in regulation of growth and development, and in energy metabolism. Our results suggest that CR can alter the expression of proteins related to the above four aspects, implying that these proteins may regulate life span of the silkworm through CR.

Characterization of recombinant expression of Bombyx mori bidensovirus ns1 using a modified vector

100%

Li G., Li M., Wang P., Hu Z., Yao Q., Tang Q., Chen K.

2014

vol. 61

issue 4

787-794

ns1 gene of Bombyx mori bidensovirus (BmBDV) consisted of 951 nucleotides encoding a deduced 316-amino aicd protein. In this study, the gene was cloned and fused in frame with a N-terminal 6×His tag under control of the polyhedrin promoter, which was transposed into the mini-attTn7 locus of a modified baculovirus vector. Transfection of Sf-9 cells with the resulting recombinant DNA was performed to prepare recombinant virus and the resultant supernatant of transfection with fluorescent signal was harvested. Western blot analysis revealed that NS1 protein was successfully expressed in Sf9 cells infected with the recombinant virus and was confirmed by LC-MS/MS analysis. Moreover, the expressed NS1 is a phosphorylated protein and the phosphorylation site is Thr-184. These results showed that the activity of BmBDV NS1 may be regulated by phosphorylation.

Cloning and partial characterization of a gene in Bombyx mori homologous to a human adiponectin receptor

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Zhu M., Chen K., Wang Y., Guo Z., Yin H., Yao Q., Chen H.

2008

vol. 55

issue 2

241-249

In this study, we report the cloning and characteristics of an adiponectin-like receptor gene from Bombyx mori (BmAdipoR) with highly conserved deduced amino-acid sequences and similar structure to the human adiponectin receptor (AdipoR). Structural analysis of the translated cDNA suggested it encoded a membrane protein with seven transmembrane domains. BmAdipoR was found to be expressed in multiple tissues and highly expressed in Malpighian tubules, fat body and testis. BmNPV (Bombyx mori nucleopolyhedrovirus) bacmid system combined with confocal microscopy revealed that BmAdipoR was targeted to the cell membrane. We also found that infection with BmNPV did not have an effect on BmAdipoR mRNA quantity in the midgut of susceptible Bombyx mori strain (306) at 48 h, but BmAdipoR mRNA quantity increased significantly at 72 h. We concluded that BmAdipoR gene was a membrane protein ubiquitously expressed in Bombyx mori tissues and that its expression was altered by treating with BmNPV.

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3 Acta Biochimica Polonica

1 2014

1 2010

1 2008

3 Chen K.

3 Yao Q.

2 Chen H.

2 Li G.

1 Guo Z.

1 Hu Z.

1 Li M.

1 Li Y.

1 Tang Q.

1 Wang L.

1 Wang P.

1 Wang Y.

1 Yin H.

1 Zhu M.

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Comparative proteomic analysis of Bombyx mori hemolymph and fat body after calorie restriction

Characterization of recombinant expression of Bombyx mori bidensovirus ns1 using a modified vector

Cloning and partial characterization of a gene in Bombyx mori homologous to a human adiponectin receptor