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STRUCTURAL AND BIOLOGICAL CHARACTERISTICS OF SELF-ORGANISING CHITOSAN HYDROGELS

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Pieklarz K., Tylman M., Modrzejewska Z., Galita G., Majsterek I.
Progress on Chemistry and Application of Chitin and its Derivatives
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2021
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vol. 26
191-199
EN
Creating innovative methods of treatment and regeneration of damaged tissues or organs is a key challenge of the twenty-first century. The aim of this study was to determine the possibility of producing and characterising the properties of self-organising chitosan hydrogels prepared with the use of chitosan lactate/chloride and disodium hydrogen phosphate dodecahydrate as a cross-linking agent. The structure and supramolecular architecture of the biomaterials were evaluated by Fourier-transform infrared spectroscopy and polarised optical microscopy. Biological studies assessed cytotoxicity by contact with a human colon adenocarcinoma cell line. The colourimetric resazurin assay showed that the obtained chitosan hydrogels are non-cytotoxic materials. Thus, self-organising biomaterials hold great promise for application in tissue engineering.
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