Full-text resources of PSJD and other databases are now available in the new Library of Science.
Visit https://bibliotekanauki.pl
Preferences help
Polish version English version Language
enabled [disable] Abstract
Number of results

Results found: 2

Number of results on page
first rewind previous Page / 1 next fast forward last

Search results

help Sort By:

help Limit search:
first rewind previous Page / 1 next fast forward last
1

Application of insect virus - baculovirus AcNPV in the expression of foreign genes

100%
Kochan G., Szewczyk B.
Biotechnologia
|
1993
|
issue 2
69-75
EN
Baculovirus vectors are very useful in producing high levels of proteins. This system yields recombinant proteins similar to their original counterparts. Expression of foreign genes occurs in infected cells which provide a suitable environment for post-translational modifications and folding of the protein product. The size of baculovirus genome allows to insert large DNA segments (up to 25kb). Constructions of recombinant baculoviruses are very simple and need minimum of viral manipulation.
2
Content available remote

Effect of tunicamycin on the biogenesis of hepatitis C virus glycoproteins

81%
Reszka N., Krol E., Patel A. H., Szewczyk B.
|
2010
|
vol. 57
|
issue 4
541-546
EN
Hepatitis C virus (HCV) infects humans, with a prevalence around 3% of population, causing acute and chronic hepatitis and hepatocellular carcinoma. We studied the effect of inhibition of glycosylation on the assembly of the HCV particle. HCV possesses two envelope glycoproteins E1 and E2 that are highly modified by N-glycans. These glycan residues are crucial for viral entry and maturation of the progeny. Here, we examined the influence of inhibition of N-glycosylation on expression of E1 and E2. Since the propagation of HCV in cell culture is limited, we used a recombinant baculovirus producing viral-like particles in insect cells. Our data showed that blocking of N-glycan transfer to the nascent polypeptide chain with the antibiotic tunicamycin resulted in the loss of E1 and E2. We also found that a dose of tunicamycin that did not influence the cell viability significantly reduced the E2 level in infected cells. The results indicate that blocking of glycosylation at an early step efficiently reduces the assembly of HCV virions. Thus, we suggest that derivatives of tunicamycin that preferentially block glycosylation of viral proteins may become potential therapeutic agents against HCV.
first rewind previous Page / 1 next fast forward last
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.