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Optical Diagnostic in Medicine - Needs and Possibilities

100%
Kasprzak J., Samsel D., Borkowska A., Kecik M.
Acta Physica Polonica A
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2011
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vol. 120
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issue 4
678-685
EN
Methods of optical imaging used in medical diagnostic (with taking the newest diagnostic techniques into consideration in particular) have been presented. Selected optical characteristics of tissues having an influence on the range of the classical methods of the optical imaging have been discussed in detail. The respond to the classical method limitations was to use scanning methods and detection techniques consisting in three-dimensional imaging of tissue structures in real time. A specific eye structure allows the wide using of these techniques in ophthalmology. Such advantages like non-invasiveness, non-contactness and high resolution (guaranteeing the safety of a patient) show a huge success of these techniques. The results of the research (conducted in many research centers) point at the wider application possibility of optical scanning method in medical diagnostic.
2
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Use of White Light Interference Methods in the Opaque Mediums Diagnostic

100%
Kasprzak J., Borkowska A., Kecik M.
Acta Physica Polonica A
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2011
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vol. 120
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issue 4
657-661
EN
The reflection, absorption and scattering influence on turbid structure has been discussed. The influence of transverse scanning method on geometrical deformations as well as issues of thickness absolute measurements and refractive index evaluation methods has been analyzed. A number of phenomena which may be the artefact source and the cause of tomogram incorrect interpretation have been pointed out.
3
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Experimental Determination of the Autocorrelation Function of Level Velocities for Microwave Networks Simulating Quantum Graphs

88%
Ławniczak M., Borkowska A., Hul O., Bauch S., Sirko L.
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EN
The autocorrelation function c(x) of level velocities is studied experimentally. The measurements were performed for microwave networks simulating quantum graphs. One and two ports measurements of the scattering matrix Ŝ necessary for determining c(x) were realized for the networks possessing 5 and 6 vertices, respectively. The network with six vertices was fully connected. In the case of the networks with five vertices, additionally to the fully connected configuration, we measured the networks without the bond connecting input/output vertices. The obtained experimental results besides the autocorrelation function of level velocities, also the nearest-neighbor spacing distribution and parametric velocities distribution are compared to the predictions of random matrix theory and numerical results.
4
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Comparison of Methods in Studies of Cell Death Mechanisms

76%
Borkowska A., Nowakowski M., Miszczyk J., Lipiec E., Wiltowska-Zuber J., Rawojć K., Kwiatek W.
Acta Physica Polonica A
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2018
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vol. 133
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issue 2
263-266
EN
While studying the influence of ionizing radiation or certain chemical agents on cells, it is crucial to not only determine cytotoxicity, but also to follow cell death mechanisms. There are different methods to screen processes of cell death and still very important question remains unanswered about differences in results that could be caused by various experimental steps in procedures. Based on literature review two protocols of cell death determination were compared. First protocol regarded collecting cells floating in medium before trypsinization and following centrifugation of them. In the second protocol floating cells were discarded and attached ones were stained and fixed. In all experiments three different untreated cell lines (A172, DU145 as cancer cell lines and in comparison, fibroblasts (FB CCL 110), as a non- cancerous cell line) were used to test applied protocols. Cells were cultured and death processes were examined at different time points up to 120 h. Compared protocols showed statistically significant differences, especially in terms of necrosis, which was higher when included floating cells from culture medium and then centrifuging them. Therefore, presented results show importance of choosing a valid experimental procedure in case of evaluating cells viability and types of cell death pathways quantitatively.
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