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Paramagnetic Centers in DOPA-Melanin-Dihydrostreptomycin Complexes

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Buszman E., Pilawa B., Zdybel M., Wrześniok D., Grzegorczyk A., Wilczok T.
Acta Physica Polonica A
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2005
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vol. 108
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issue 2
353-356
EN
DOPA-melanin-dihydrostreptomycin complexes with drug concentrations 1×10^{-4}-1×10^{-2} M were examined by the use of electron paramagnetic resonance spectroscopy at X-band (9.3 GHz). Dihydrostreptomycin was chosen for studies, because this aminoglycoside antibiotic causes strong toxic effects in organism. It was stated that dihydrostreptomycin generates o-semiquinone free radicals with g=2.0038 in melanin. Free radicals formation increases with increase in the antibiotic concentration. Changes of EPR lines with microwave powers pointed out that slow spin-lattice relaxation processes exist in DOPA-melanin and in its complexes with dihydrostreptomycin. The measured EPR lines were homogeneously broadened.
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