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Isolation and Identification of Potential Pathogenic Bacteria in Living Carp (Cyprinus carpio Linnaeus, 1758) Sold in Supermarkets in Cimahi City, Java

100%
Geraldine A. R., Rosidah R., Herawati H., Bioshina I. B.
World News of Natural Sciences
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2020
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vol. 32
21-35
EN
This research was conducted with the aim to analyze the presence of potential pathogenic bacteria in carp that are sold live in supermarkets in the city of Cimahi and to find out the species of these bacteria. Fish samples were obtained from two supermarkets in Cimahi City, Transmart and Superindo. From each supermarket three fish samples were taken once a week and repeated three times. Bacteria were isolated from several parts of the fish body namely body surface mucus, gills, liver, and kidneys. The results of isolation from each target organ were biochemically tested to determine the species of bacteria. Potential pathogenic bacteria found in carp from this research are Aeromonas hydrophila, Aeromonas sobria, Aeromonas schubertii, Aeromonas media, Aeromonas caviae, Aeromonas ecrenophila, Pseudomonas stutzeri, Pseudomonas pseudoalcaligenes, Pseudomonas aeruginosa, Aeromonas caviae, Aeromonas ecrenophila, Pseudomonas stutzeri, Pseudomonas pseudoalcaligenes, Pseudomonas aeruginosa, Plesiomonas shigelloides, Bacillus megaterium, Bacillus thuringiensis, Neisseria mucosa, Citrobacter freundii, Serratia marcescens, Staphylococcus epidermidis, Micrococcus luteus, Enterobacter aerogenes, and Enterobacter sakazakii.
2
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The effectivity of metabolite secondary extract of bacteria associated with sea grass (Cymodocea rotundata) for Vibriosis treatment in tiger shrimp (Penaeus monodon)

100%
Lili W., Maulana G., Astuty S., Rosidah R.
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vol. 26
96-115
EN
The natural occurrence of the shrimp (Penaeus monodon Fabricius, 1798) and seagrass (Cymodocea rotundata Asch. & Schweinf.) is found in the area of Indian Ocean, Indonesia, for central Pacific. Vibriosis can cause death in larvae, juvenile and adult shrimp almost close to 100%. Antibacterial substances from secondary metabolites are produced by plants and animals to reduce the use of synthetic antibiotics. The purpose of this research was to determine the ability of secondary metabolites contained in the bacterial extract of the association of sea grass Cymodocea rotundata (isolate code BA.1) to treat vibriosis in tiger shrimp (Penaeus monodon). This research was conducted at the Laboratory of Integrated Biotechnology and Laboratory of Aquaculture, Fisheries and Marine Sciences Faculty, Universitas Padjadajaran, and at the Brackish and Southern Ocean Aquaculture Development Center Pangandaran, from June to September 2018. The research method used was experimental, with completely randomized design (CRD) consisting of five treatments with three replications including; control treatment (concentration of 0 mg·L–1), 150 mg.L–1, 300 mg·L–1, 450 mg·L–1 and 600 mg·L–1. Observations made include clinical symptoms (morphology, behavior) and survival rate. Clinical symptoms of infected tiger shrimp during the in vivo test were red spots on the abdomen and necrosis of some leg and tail segments. The behavior of tiger shrimp mostly shows less responsive movements to fish-feed on the first day, and showing responsive and active behavior on the fifth day. In vivo test results for 14 days showed that the highest survival rate of 51.67% was in case of the addition of antibacterial extracts of BA.1 with a concentration of 300 mg·L–1.
3
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Effect of Types Isolated Lactic Acid Bacteria on Hematocrit and Differential Leukocytes Fingerling Common Carp (Cyprinus carpio L.) Infected with Aeromonas hydrophila bacteria

100%
Shabirah A., Rosidah R., Mulyani Y., Lili W.
World News of Natural Sciences
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2019
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vol. 24
22-35
EN
This study aims to see the condition of hematocrit levels and differential leukosit after immersion in LAB isolated and after in vivo, using Aeromonas hydrophila bacterium. LAB isolated were obtained from the organs of the common carp intestine. The study used a Completely Randomized Design (RAL) consisting of four treatments and three replications. The treatment used was immersion of test fish for 24 hours in several isolates of LAB with a density of 108, namely A: without LAB isolate (control), B: isolate CcB7, C: isolate CcB8, D: isolate CcB15. Immersion was done three times, with a frequency of seven days. Parameters observed included hematocrit levels, leukocyte differentiation and survival rate. The results showed that immersion in LAB isolate CcB15 will enhance the differential leukocytes and hematocrit levels that serve as indicators of carp body resistance, which is shown by high levels of white and red blood cells.
4
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Immune performances of sangkuriang catfish (Clarias gariepinus) with addition of potassium diformate on feed

100%
Yustiati A., Nadiyah N. A., Suryadi I. B. B., Rosidah R.
World News of Natural Sciences
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2019
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vol. 25
113-129
EN
This research aims to determine the effective dosage of potassium diformate (KDF) in feed to enhance sangkuriang catfish immune performances. The research was conducted on November - January 2019, at Aquaculture Laboratory Building No. 4 Faculty of Fisheries and Marine Sciences Universitas Padjadjaran. This study used an experimental method of Completely Randomized Design (CRD), with four treatments and three replications. The treatments are the addition of potassium diformate into feed with doses A (0%), B (0.5%), C (0.7%) and D (0.9%). Observed parameters are clinical symptoms, total leukocyte count, total erythrocyte count, specific growth rate, survival rate and water quality. The results showed that 0.5% KDF feed produced the highest survival rate of 85% and the highest specific growth rate of 3.59% after 40 days. Furthermore, due to treatment, total erythrocyte count increased from 2.09×106 cells/mm3, to 2.10×106 cells/mm3 (8.1%) and total leukocyte count increased from 7.1×104 cells/mm3, to 10.7×104 cells/mm3 (42.81%). The addition of 0.5% KDF in feed is effective in improving the immune performances of sangkuriang catfish challenged by A. hydrophila, with the highest survival rate of 97%. Moreover, recovery of total leukocyte count and total erythrocyte count was faster than other treatments as of day 7th, with a value 1.97×106 sel/mm3and 12.60×104 sel/mm3. It also resulted in the lowest gross clinical signs and the fastest recovering.
5
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Effect of Adding Pumpkin Flour and Carrot Flour on Changes in Color Intensity of Goldfish (Carassius auratus L.) Strain Oranda

88%
Ahlam R. N., Lili W., Hamdani H., Rosidah R., Bangkit I.
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vol. 26
52-60
EN
Goldfish is one of the most famous ornamental fish because of its body shape and color which was interesting to be maintained. The purpose of this research was to find the optimum dose of pumpkin and carrot flour combination on artificial feed to increase color brightness of goldfish. Complete Randomized Design with four treatments and four replications were used in the experiment. For the treatments, pumpkin flour 15%, pumpkin flour 7.5% with carrot flour 2.5% and carrot flour 5%, were studied. The parameters observed were color intensity, survival rate, and water quality. The results showed that supplementation of combination of pumpkin flour 7.5% with carrot flour 2.5% gave the highest color intensity of goldfish with TCF code 0815 and score 2.11 with 100% survival rate. Water qualities during the research were still in optimal condition.
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